ARG41026
anti-WARS / TrpRS antibody
anti-WARS / TrpRS antibody for Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes WARS / TrpRS |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | WARS / TrpRS |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-270 of Human WARS (NP_776049.1). |
| 偶联标记 | Un-conjugated |
| 別名 | GAMMA-2; Tryptophanyl-tRNA synthetase; Tryptophan--tRNA ligase, cytoplasmic; hWRS; EC 6.1.1.2; TrpRS; Interferon-induced protein 53; IFP53; IFI53 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | MCF7 | ||||
| 实际分子量 | 55 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | WARS |
| 全名 | tryptophanyl-tRNA synthetase |
| 背景介绍 | Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression. [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 预测分子量 | 53 kDa |
| 翻译后修饰 | Proteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG41026 anti-WARS / TrpRS antibody WB image
Western blot: 25 µg of MCF7 cell lysate stained with ARG41026 anti-WARS / TrpRS antibody at 1:1000 dilution.
