ARG40970
anti-Vitronectin antibody
anti-Vitronectin antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Vitronectin |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Vitronectin |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide between aa. 65-93 of Human Vitronectin. |
| 偶联标记 | Un-conjugated |
| 別名 | Vitronectin; V75; VN; Serum-spreading factor; S-protein; VNT |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Caco2 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | VTN |
| 全名 | vitronectin |
| 背景介绍 | The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. [provided by RefSeq, Jul 2008] |
| 生物功能 | Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway. Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity. [UniProt] |
| 细胞定位 | Secreted, extracellular space. [UniProt] |
| 预测分子量 | 54 kDa |
| 翻译后修饰 | Sulfated on 2 tyrosine residues. N- and O-glycosylated. Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading. It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both. Phosphorylation sites are present in the extracellular medium. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG40970 anti-Vitronectin antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human lung carcinoma tissue stained with ARG40970 anti-Vitronectin antibody.
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ARG40970 anti-Vitronectin antibody WB image
Western blot: 20 µg of Caco2 cell lysate stained with ARG40970 anti-Vitronectin antibody at 1:2000 dilution.
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ARG40970 anti-Vitronectin antibody FACS image
Flow Cytometry: MCF7 cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by ARG40970 anti-Vitronectin antibody (right histogram) at 1:25 dilution for 60 min at 37°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (left histogram) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.
