ARG66958
anti-Vinculin antibody
anti-Vinculin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody; Loading Control antibody for cytoplasmic fraction; High MW loading control antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Vinculin |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Vinculin |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the middle region of Human Vinculin. |
| 偶联标记 | Un-conjugated |
| 別名 | HEL114; Metavinculin; CMH15; Vinculin; CMD1W; MV; MVCL |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 125 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | 100 mM Tris Glycine (pH 7.0), 0.025% ProClin 300 and 20% Glycerol. |
| 抗菌剂 | 0.025% ProClin 300 |
| 稳定剂 | 20% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | VCL |
| 全名 | vinculin |
| 背景介绍 | Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008] |
| 生物功能 | Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody; Loading Control antibody for cytoplasmic fraction; High MW loading control antibody |
| 预测分子量 | 124 kDa |
| 翻译后修饰 | Phosphorylated; on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques (By similarity). Acetylated; mainly by myristic acid but also by a small amount of palmitic acid. |
检测图片 (5) Click the Picture to Zoom In
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ARG66958 anti-Vinculin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded mouse testis tissue stained with ARG66958 anti-Vinculin antibody at 1:300 dilution.
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ARG66958 anti-Vinculin antibody ICC/IF image image
Immunofluorescence: Formalin-fixed DBTRG cells were permeabilized with 0.1% NP-40 in TBS for 10 minutes and blocked with 5% BSA-PBS for 30 minutes at room temperature. Cells were stained ARG66958 anti-Vinculin antibody at 1:200 dilution.
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ARG66958 anti-Vinculin antibody WB image image
Western blot: Mouse liver stained withARG66958 anti-Vinculin antibody at 1:3000 dilution, overnight at 4°C.
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ARG66958 anti-Vinculin antibody ICC/IF image image
Immunofluorescence: Formalin-fixed HUVEC cells were permeabilized with 0.1% NP-40 in TBS for 10 minutes and blocked with 5% BSA-PBS for 30 minutes at room temperature. Cells were stained ARG66958 anti-Vinculin antibody at 1:200 dilution.
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ARG66958 anti-Vinculin antibody WB image image
Western blot: HepG2 and HeLa cells stained withARG66958 anti-Vinculin antibody at 1:3000 dilution, overnight at 4°C.
