ARG57063
anti-VTA1 antibody [14G10]
anti-VTA1 antibody [14G10] for Flow cytometry,ICC/IF,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [14G10] recognizes VTA1 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 14G10 |
| 同位型 | IgG1, kappa |
| 靶点名称 | VTA1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-307 of Human VTA1. |
| 偶联标记 | Un-conjugated |
| 別名 | DRG1; HSPC228; Vacuolar protein sorting-associated protein VTA1 homolog; SKD1-binding protein 1; LYST-interacting protein 5; C6orf55; LIP5; SBP1; My012; Dopamine-responsive gene 1 protein; DRG-1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9NP79 Human Vacuolar protein sorting-associated protein VTA1 homolog |
|---|---|
| 基因名称 | VTA1 |
| 全名 | vesicle (multivesicular body) trafficking 1 |
| 背景介绍 | C6ORF55 encodes a protein involved in trafficking of the multivesicular body, an endosomal compartment involved in sorting membrane proteins for degradation in lysosomes (Ward et al., 2005 [PubMed 15644320]).[supplied by OMIM, Mar 2008] |
| 生物功能 | Involved in the endosomal multivesicular bodies (MVB) pathway. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. Thought to be a cofactor of VPS4A/B, which catalyzes disassembles membrane-associated ESCRT-III assemblies. Involved in the sorting and down-regulation of EGFR (By similarity). Involved in HIV-1 budding. [UniProt] |
| 预测分子量 | 34 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG57063 anti-VTA1 antibody [14G10] ICC/IF image
Immunoflorescense: Hep3B cell line stained with ARG57063 anti-VTA1 antibody [14G10] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57063 anti-VTA1 antibody [14G10] FACS image
Flow Cytometry: Hep3B cell line stained with ARG57063 anti-VTA1 antibody [14G10] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
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ARG57063 anti-VTA1 antibody [14G10] WB image
Western blot: 40 µg of 1) HepG2 cell lysate, 2) 293T cell lysate, 3) HeLa cell lysate, 4) MCF7 cell lysate, 5) A549 cell lysate, 6) Jurkat cell lysate, 7) K562 cell lysate, 8) LnCaP cell lysate, 9) U937 cell lysate, 10) A431 cell lysate stained with ARG57063 anti-VTA1 antibody [14G10] at 1:1000.
