anti-VEGF antibody [VG-1]
anti-VEGF antibody [VG-1] for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Dog,Human,Mouse,Rabbit,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
Mouse Monoclonal antibody [VG-1] recognizes VEGF
|反应物种||Hu, Ms, Rat, Dog, Rb|
|应用||ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB|
|特异性||Detects the 121, 165 and 189 VEGF isoforms in fixed specimens.|
|抗原||Recombinant VEGF 189 protein.|
|別名||MVCD1; Vascular permeability factor; VEGF-A; VPF; VEGF; Vascular endothelial growth factor A|
|应用说明||WB: Suggested blocking buffer: 3% milk blocking for 1 hr at RT (better than at 4°C, overnight). Recommend primary and secondary dilution buffer: TBST.
IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0 (recommended) or citrate buffer pH6.0.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|阳性对照||Corpus luteum, VEGF165 recombinant protein, astrocytoma or hemoangiosarcoma.|
|纯化||Purified by affinity chromatography.|
|缓冲液||PBS and 0.02% Sodium azide|
|抗菌剂||0.02% Sodium azide|
|存放说明||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|注意事项||For laboratory research only, not for drug, diagnostic or other use.|
|全名||Vascular endothelial growth factor A|
|背景介绍||VEGF is an angiogenic growth factor and a prognostic indicator for cancer when detected in serum. VG-1 is useful for studies of VEGF and angiogenesis in human pathological material.|
|生物功能||Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. [Uniprot]|
VEGF antibodies; VEGF ELISA Kits; VEGF Duos / Panels; VEGF recombinant proteins; Anti-Mouse IgG secondary antibodies;
The role of HDGF in tumor angiogenesis
|研究领域||Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody|
|预测分子量||16 - 45 kDa|
检测图片 (3) Click the Picture to Zoom In
ARG10513 anti-VEGF antibody [VG-1] IHC-P image
Immunohistochemistry: Mouse formula stained with ARG10513 anti-VEGF antibody [VG-1].
From Chenjie Xia et al. J Orthop Surg Res. (2023), doi: 10.1186/s13018-023-03696-7, Fig. 7A.
ARG10513 anti-VEGF antibody [VG-1] ICC/IF image
Immunofluorescence: Hamstring tenocytes treated with ARG10513 anti-VEGF antibody [VG-1].
From Cheng-Chang Lu et al. Bone Joint Res ., (2023) doi: doi.org/10.1302/2046-3758.121.BJR-2021-0434.R2, Fig. 6C.
ARG10513 anti-VEGF antibody [VG-1] WB image
Western blot: Mouse gastrocnemius stained with ARG10513 anti-VEGF antibody [VG-1].
From Yong-Qing Do et al. Theranostics. (2020), doi: 10.7150/thno.39320, Fig. 2A.