ARG20567
anti-VE Cadherin phospho (Tyr685) antibody
anti-VE Cadherin phospho (Tyr685) antibody for Western blot and Human
Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes VE Cadherin phospho (Tyr685) |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat |
| 应用 | WB |
| 特异性 | The purified antibody detects a ~140 kDa band corresponding to VE-cadherin in western blots of human endothelial cells treated with pervanadate, and this band is not detected in untreated cells or after alkaline phosphatase treatment. This sequence is not conserved in other cadherins. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | VE Cadherin |
| 抗原物种 | Human |
| 抗原 | Phosphospecific peptide (coupled to carrier protein) around Tyr685 of Human VE-cadherin. |
| 偶联标记 | Un-conjugated |
| 別名 | 7B4 antigen; 7B4; Cadherin-5; VE-cadherin; CD144; CD antigen CD144; Vascular endothelial cadherin |
应用说明
| 应用建议 |
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| 应用说明 | WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | 140 kDa |
属性
| 纯化 | This antibody was pre-adsorbed with an unrelated phospho-tyrosine peptide and unphosphorylated VE-cadherin (Tyr685) peptide then affinity purified with immunogen peptide. |
|---|---|
| 缓冲液 | PBS, 50% Glycerol, 1 mg/ml BSA and 0.05% Sodium azide |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 50% Glycerol, 1 mg/ml BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CDH5 |
| 全名 | cadherin 5, type 2 (vascular endothelium) |
| 背景介绍 | This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq, Jul 2008] |
| 生物功能 | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. [UniProt] |
| 研究领域 | Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody |
| 预测分子量 | 88 kDa (unmodified); 90 - 140 kDa (glycosylated) |
| 翻译后修饰 | Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB (By similarity). O-glycosylated. |
检测图片 (1) Click the Picture to Zoom In
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ARG20567 anti-VE Cadherin phospho (Tyr685) antibody WB image
Western blot: Human umbilical vein endothelial cells stimulated with pervanadate (1 mM) for 30 min. then the blots were untreated (Lanes 1 & 3) or treated with alkaline phosphatase (Lanes 2 & 4). The blots were stained with ARG20567 anti-VE Cadherin phospho (Tyr685) antibody (Lanes 1 & 2) or Mouse monoclonal anti-VE-cadherin antibody (Lanes 3 & 4).
