ARG10663
anti-VASP antibody
anti-VASP antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Pig
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes VASP |
|---|---|
| 反应物种 | Hu, Ms, Pig |
| 应用 | ICC/IF, IP, WB |
| 特异性 | The antibody recognizes both the 46 kDa (Ser-157 dephospho) and 50 kDa (Ser-157 phospho) form of VASP. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | VASP |
| 抗原物种 | Human |
| 抗原 | His-tagged recombinant full-length Human VASP protein. |
| 偶联标记 | Un-conjugated |
| 別名 | VASP; Vasodilator-stimulated phosphoprotein |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Human platelet protein (lysate). |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.02% Sodium azide and 1 mg/ml BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 1 mg/ml BSA |
| 浓度 | 0.25 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P50552 Human Vasodilator-stimulated phosphoprotein Swiss-port # P70460 Mouse Vasodilator-stimulated phosphoprotein |
|---|---|
| 基因名称 | VASP |
| 全名 | vasodilator-stimulated phosphoprotein |
| 背景介绍 | Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq, Jul 2008] |
| 生物功能 | Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. [UniProt] |
| 预测分子量 | 46 kDa (Ser157 dephospho); 50 kDa (Ser157 phospho) |
| 翻译后修饰 | Major substrate for cAMP-dependent (PKA) and cGMP-dependent protein kinase (PKG) in platelets. The preferred site for PKA is Ser-157, the preferred site for PKG/PRKG1, Ser-239. In ADP-activated platelets, phosphorylation by PKA or PKG on Ser-157 leads to fibrinogen receptor inhibition. Phosphorylation on Thr-278 requires prior phosphorylation on Ser-157 and Ser-239. In response to phorbol ester (PMA) stimulation, phosphorylated by PKC/PRKCA. In response to thrombin, phosphorylated by both PKC and ROCK1. Phosphorylation at Thr-278 by AMPK does not require prior phosphorylation at Ser-157 or Ser-239. Phosphorylation at Ser-157 by PKA is required for localization to the tight junctions in epithelial cells. Phosphorylation modulates F-actin binding, actin filament elongation and platelet activation. Phosphorylation at Ser-322 by AMPK also alters actin filament binding. Carbon monoxide (CO) promotes phosphorylation at Ser-157, while nitric oxide (NO) promotes phosphorylation at Ser-157, but also at Ser-239. Response to NO and CO is blunted in platelets from diabetic patients, and VASP is not phosphorylated efficiently at Ser-157 and Ser-239. |
检测图片 (3) Click the Picture to Zoom In
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ARG10663 anti-VASP antibody ICC/IF image
Immunofluorescence: Focal contacts and actin filaments of a Human skin fibroblast stained with ARG10663 anti-VASP antibody.
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ARG10663 anti-VASP antibody WB image
Western blot: Monitoring cAMP-/cGMP-dependent protein kinase activity. The blots were stained with ARG10663 anti-VASP antibody. The shift from 46 to 50 kDa indicates Ser-157 phosphorylation.
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ARG10663 anti-VASP antibody WB image
Western blot: Human platelets (left lane) and Human skin fibroblasts (right lane) stained with ARG10663 anti-VASP antibody.
