ARG62340
anti-V5 tag antibody [V5.E10]
anti-V5 tag antibody [V5.E10] for Dot blot,ELISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Other
Controls and Markers antibody; Gene Regulation antibody
概述
| 产品描述 | Mouse Monoclonal antibody [V5.E10] recognizes V5 tag |
|---|---|
| 反应物种 | Other |
| 应用 | Dot, ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| 特异性 | Recognizes V5-tagged fusion proteins |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | V5.E10 |
| 同位型 | IgG1 |
| 靶点名称 | V5 tag |
| 抗原物种 | Others |
| 抗原 | GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH. |
| 偶联标记 | Un-conjugated |
应用说明
| 应用建议 |
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| 应用说明 | The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Protein A Purified |
| 纯化说明 | Protein A affinity chromatography from culture supernatant of hybridoma cells grown in a bioreactor. |
| 缓冲液 | 10mM PBS (pH 7.2) and 0.05% Sodium azide |
| 抗菌剂 | 0.05% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 背景介绍 | The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). Both sequence is rarely be found in other proteins and can be used for protein analysis including binding assay, functional analysis and tracing the protein location in cells when the specific antibodies for the protein-of-interest are unavailable. |
|---|---|
| 产品亮点 | Related products: V5 tag antibodies; Anti-Mouse IgG secondary antibodies; |
| 研究领域 | Controls and Markers antibody; Gene Regulation antibody |
检测图片 (2) Click the Picture to Zoom In
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ARG62340 anti-V5 tag antibody [V5.E10] WB image
Western blot: 10 µg of 293T and 293T + myc-V5-mCherry cell lysates stained with ARG62340 anti-V5 tag antibody [V5.E10] at 1:1000 dilution.
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ARG62340 anti-V5 tag antibody [V5.E10] IHC image
Immunohistochemistry: Zebrafish Embryos fixed with 0.4% Triton X-100 containing 4% paraformaldehyde and then stained with ARG62340 anti-V5 tag antibody [V5.E10] at 1:250 dilution.
客户反馈
Excellent
anti-V5 tag antibody [V5.E10]
Application：WB
Sample：293T and 293T + myc-V5-mCherry
Sample Loading Amount：10 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
