ARG40759
anti-USO1 antibody
anti-USO1 antibody for ICC/IF,Western blot and Human,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes USO1 |
|---|---|
| 反应物种 | Hu, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | USO1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 663-962 of Human USO1 (NP_003706.2). |
| 偶联标记 | Un-conjugated |
| 別名 | Protein USO1 homolog; VDP; TAP; Transcytosis-associated protein; Vesicle-docking protein; P115; General vesicular transport factor p115 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | SGC7901 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O60763 Human General vesicular transport factor p115 Swiss-port # P41542 Rat General vesicular transport factor p115 |
|---|---|
| 基因名称 | USO1 |
| 全名 | USO1 vesicle transport factor |
| 背景介绍 | The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] |
| 生物功能 | General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity (By similarity). [UniProt] |
| 细胞定位 | Cytoplasm, cytosol. Golgi apparatus membrane; Peripheral membrane protein. Note=Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes. [UniProt] |
| 预测分子量 | 108 kDa |
| 翻译后修饰 | Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG40759 anti-USO1 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG40759 anti-USO1 antibody at 1:200 dilution.
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ARG40759 anti-USO1 antibody WB image
Western blot: 25 µg of SGC7901 cell lysate stained with ARG40759 anti-USO1 antibody at 1:1000 dilution.
