ARG54991
anti-UNC13B antibody
anti-UNC13B antibody for Flow cytometry,Western blot and Human
Neuroscience antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes UNC13B |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms |
| 应用 | FACS, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | UNC13B |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 1062-1091 (Center) of Human UNC13B. |
| 偶联标记 | Un-conjugated |
| 別名 | munc13; Protein unc-13 homolog B; UNC13; Munc13-2; Unc13h2; MUNC13 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | 293 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | UNC13B |
| 全名 | unc-13 homolog B (C. elegans) |
| 背景介绍 | This gene is expressed in the kidney cortical epithelial cells and is upregulated by hyperglycemia. The encoded protein shares a high level of similarity to the rat homolog, and contains 3 C2 domains and a diacylglycerol-binding C1 domain. Hyperglycemia increases the levels of diacylglycerol, which has been shown to induce apoptosis in cells transfected with this gene and thus contribute to the renal cell complications of hyperglycemia. Studies in other species also indicate a role for this protein in the priming step of synaptic vesicle exocytosis. [provided by RefSeq, Jul 2008] |
| 生物功能 | Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-depending refilling of readily releasable vesicle pool (RRP). Essential for synaptic vesicle maturation in a subset of excitatory/glutamatergic but not inhibitory/GABA-mediated synapses (By similarity). [UniProt] |
| 细胞定位 | Cytoplasm. Membrane; Peripheral membrane protein. Cell membrane. Cell junction, synapse Note=Localized to synapses. Translocated to the plasma membrane in response to phorbol ester binding (By similarity). |
| 研究领域 | Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 181 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG54991 anti-UNC13B antibody WB image
Western blot: 35 µg of 293 cell lysate stained with ARG54991 anti-UNC13B antibody.
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ARG54991 anti-UNC13B antibody FACS image
Flow Cytometry: 293 cells stained with ARG54991 anti-UNC13B antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.
