ARG54949
anti-ULK3 antibody
anti-ULK3 antibody for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ULK3 |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA, ICC/IF, IHC-P, WB |
| 特异性 | ULK3 antibody is human specific. Multiple isoforms of ULK3 are known to exist. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ULK3 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide (19 aa) within aa. 230-280 of Human ULK3. |
| 偶联标记 | Un-conjugated |
| 別名 | EC 2.7.11.1; Unc-51-like kinase 3; Serine/threonine-protein kinase ULK3 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | Human Brain Tissue Lysate |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q6PHR2 Human Serine/threonine-protein kinase ULK3 |
|---|---|
| 基因名称 | ULK3 |
| 全名 | unc-51 like kinase 3 |
| 背景介绍 | ULK3 belongs to the Ser/Thr protein kinase superfamily and plays a role in the ATP-dependent phosphorylation of target proteins. Knockout of ULK genes results in a severe defect in the autophagy pathway. ULK3, like the other Unc-51-like kinases such as ULK1, ULK2 and ULK4, is highly conserved among eukaryotes. ULK3 has been shown to be a positive regulator of the Hedgehog signaling pathway by enhancing GLI1 and GLI2 transcriptional activity. Furthermore, ULK3 can also interact with SUFU, a protein required for the negative regulation of GLI proteins; this interaction blocks the autophosphorylation of ULK3 and blocks its ability to regulate the GLI proteins. |
| 生物功能 | Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy. [UniProt] |
| 研究领域 | Signaling Transduction antibody |
| 预测分子量 | 53 kDa |
| 翻译后修饰 | Autophosphorylated. Autophosphorylation is blocked by interaction with SUFU. |
检测图片 (3) Click the Picture to Zoom In
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ARG54949 anti-ULK3 antibody WB image
Western blot: human brain tissue lysate stained with ARG54949 anti-ULK3 antibody at (A) 0.5 and (B) 1 ug/ml dilution.
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ARG54949 anti-ULK3 antibody IHC image
Immunohistochemistry: ULK3 in human brain tissue stained with ARG54949 anti-ULK3 antibody at 20 ug/ml dilution.
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ARG54949 anti-ULK3 antibody IHC image
Immunohistochemistry: ULK3 in human brain tissue stained with ARG54949 anti-ULK3 antibody at 5 ug/ml dilution.
