ARG54990
anti-ULK3 antibody [1404CT148.89.25]
anti-ULK3 antibody [1404CT148.89.25] for Western blot and Human,Mouse,Rat
Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes ULK3 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1404CT148.89.25 |
| 同位型 | IgG1, kappa |
| 靶点名称 | ULK3 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 435-468 of Human ULK3. |
| 偶联标记 | Un-conjugated |
| 別名 | EC 2.7.11.1; Unc-51-like kinase 3; Serine/threonine-protein kinase ULK3 |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | HeLa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ULK3 |
| 全名 | unc-51 like kinase 3 |
| 生物功能 | Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy. [UniProt] |
| 细胞定位 | Cytoplasm. Note=Localizes to pre-autophagosomal structure during cellular senescence |
| 研究领域 | Signaling Transduction antibody |
| 预测分子量 | 53 kDa |
| 翻译后修饰 | Autophosphorylated. Autophosphorylation is blocked by interaction with SUFU. |
检测图片 (1) Click the Picture to Zoom In
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ARG54990 anti-ULK3 antibody WB image
Western blot: 35 µg of HeLa cell lysate stained with ARG54990 anti-ULK3 antibody at 1:1000 dilution.
