ARG59484
anti-UHRF2 / NIRF antibody
anti-UHRF2 / NIRF antibody for Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes UHRF2 / NIRF |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow, Dog, Gpig, Hrs, Rb |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | UHRF2 / NIRF |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the N-terminal region of Human UHRF2 / NIRF. (within the following region: TNKLDSVPSTSNSDCVAADEDVIYHIQYDEYPESGTLEMNVKDLRPRART) |
| 偶联标记 | Un-conjugated |
| 別名 | Nuclear zinc finger protein Np97; EC 6.3.2.-; Ubiquitin-like PHD and RING finger domain-containing protein 2; RING finger protein 107; Nuclear protein 97; Np95-like RING finger protein; RNF107; NIRF; URF2; Np95/ICBP90-like RING finger protein; Ubiquitin-like-containing PHD and RING finger domains protein 2; E3 ubiquitin-protein ligase UHRF2 |
应用说明
| 预测反应性说明 | Predicted Homology Based On Immunogen Sequence: Cow: 86%; Dog: 86%; Guinea pig: 93%; Horse: 86%; Mouse: 86%; Rabbit: 100%; Rat: 93% | ||||
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| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | Jurkat | ||||
| 实际分子量 | ~ 85 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS, 0.09% (w/v) Sodium azide and 2% Sucrose. |
| 抗菌剂 | 0.09% (w/v) Sodium azide |
| 稳定剂 | 2% Sucrose |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | UHRF2 |
| 全名 | ubiquitin-like with PHD and ring finger domains 2, E3 ubiquitin protein ligase |
| 背景介绍 | This gene encodes a nuclear protein which is involved in cell-cycle regulation. The encoded protein is a ubiquitin-ligase capable of ubiquinating PCNP (PEST-containing nuclear protein), and together they may play a role in tumorigenesis. The encoded protein contains an NIRF_N domain, a PHD finger, a set- and ring-associated (SRA) domain, and a RING finger domain and several of these domains have been shown to be essential for the regulation of cell proliferation. This protein may also have a role in intranuclear degradation of polyglutamine aggregates. Alternative splicing results in multiple transcript variants some of which are non-protein coding. [provided by RefSeq, Feb 2012] |
| 生物功能 | E3 ubiquitin-protein ligase that is an intermolecular hub protein in the cell cycle network. Through cooperative DNA and histone binding, may contribute to a tighter epigenetic control of gene expression in differentiated cells. Ubiquitinates cyclins, CCND1 and CCNE1, in an apparently phosphorylation-independent manner and induces G1 arrest. Also ubiquitinates PCNP leading to its degradation by the proteasome. E3 SUMO-, but not ubiquitin-, protein ligase for ZNF131. [UniProt] |
| 细胞定位 | Nucleus. Note=Enriched at pericentric heterochromatin (PH). This localization is dependent on the interaction with H3K9me3 (By similarity). [UniProt] |
| 预测分子量 | 90 kDa |
| 翻译后修饰 | May be autoubiquitinated; which may lead to proteasomal degradation. Phosphorylated. Phosphorylation may be mediated by CDK2. Autosumoylated. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG59484 anti-UHRF2 / NIRF antibody WB image
Western blot: Jurkat cell lysate stained with ARG59484 anti-UHRF2 / NIRF antibody at 0.2 - 1 µg/ml dilution.
