ARG64193
anti-UCP2 antibody
anti-UCP2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Metabolism antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes UCP2 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 预测物种 | Dog |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | UCP2 |
| 抗原物种 | Human |
| 抗原 | C-DSVKQFYTKGSEH |
| 偶联标记 | Un-conjugated |
| 別名 | UCPH; Solute carrier family 25 member 8; SLC25A8; BMIQ4; Mitochondrial uncoupling protein 2; UCP 2 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed in many tissues, with the greatest expression in skeletal muscle. It is thought to play a role in nonshivering thermogenesis, obesity and diabetes. Chromosomal order is 5'-UCP3-UCP2-3'. [provided by RefSeq, Jul 2008] |
| 研究领域 | Cancer antibody; Metabolism antibody |
| 预测分子量 | 33 kDa |
检测图片 (6) Click the Picture to Zoom In
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ARG64193 anti-UCP2 antibody WB image
Western blot: Human Liver lysate (35 µg protein in RIPA buffer) stained with ARG64193 anti-UCP2 antibody at 0.05 µg/ml dilution.
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ARG64193 anti-UCP2 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed MCF7 cells permeabilized with 0.15% Triton. Cells were stained with ARG64193 anti-UCP2 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG64193 anti-UCP2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64193 anti-UCP2 antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG64193 anti-UCP2 antibody WB image
Western blot: 35 µg of Rat adipose (A) and Mouse spleen (B) lysates (in RIPA buffer) stained with ARG64193 anti-UCP2 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
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ARG64193 anti-UCP2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human skeletal muscle tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64193 anti-UCP2 antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG64193 anti-UCP2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human adipocytes. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64193 anti-UCP2 antibody at 3.75 µg/ml dilution followed by AP-staining.
