ARG63944
anti-UCP1 antibody
anti-UCP1 antibody for Western blot and Rat
Cancer antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes UCP1 |
|---|---|
| 反应物种 | Rat |
| 预测物种 | Hu, Ms, Dog |
| 应用 | WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | UCP1 |
| 抗原物种 | Human |
| 抗原 | C-EQLKRELSKSRQ |
| 偶联标记 | Un-conjugated |
| 別名 | UCP; SLC25A7; Thermogenin; Mitochondrial brown fat uncoupling protein 1; Solute carrier family 25 member 7; UCP 1 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P04633 Rat Mitochondrial brown fat uncoupling protein 1 |
|---|---|
| 背景介绍 | Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat. [provided by RefSeq, Jul 2008] |
| 产品亮点 | Related products: UCP1 antibodies; Anti-Goat IgG secondary antibodies; Related news: Has “Obesity gene” been found? |
| 研究领域 | Cancer antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 33 kDa |
| 翻译后修饰 | May undergo sulfenylation upon cold exposure. May increase the sensitivity of UCP1 thermogenic function to the activation by noradrenaline probably through structural effects. May undergo ubiquitin-mediated proteasomal degradation. |
检测图片 (2) Click the Picture to Zoom In
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ARG63944 anti-UCP1 antibody WB image
Western Blot: Human Adipose lysate (35 µg protein in RIPA buffer) stained with ARG63944 anti-UCP1 antibody at 1 µg/ml dilution.
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ARG63944 anti-UCP1 antibody WB image
Western blot: 35 µg of Rat adipose (A) and Rat skeletal muscle (B) lysates (in RIPA buffer) stained with ARG63944 anti-UCP1 antibody at 1 µg/ml (A) and 0.5 µg/ml (B) dilutions and incubated at RT for 1 hour.
