ARG55814
anti-UCHL1 / PGP9.5 antibody [346CT2.2.1]
anti-UCHL1 / PGP9.5 antibody [346CT2.2.1] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Mouse Monoclonal antibody recognizes UCHL1 / PGP9.5 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 346CT2.2.1 |
| 同位型 | IgG1, kappa |
| 靶点名称 | UCHL1 / PGP9.5 |
| 抗原物种 | Human |
| 抗原 | UCHL1 recombinant protein. |
| 偶联标记 | Un-conjugated |
| 別名 | PGP95; UCH-L1; PGP9.5; PARK5; Ubiquitin thioesterase L1; HEL-117; Neuron cytoplasmic protein 9.5; Uch-L1; EC 6.-.-.-; PGP 9.5; Ubiquitin carboxyl-terminal hydrolase isozyme L1; NDGOA; EC 3.4.19.12 |
应用说明
| 应用建议 |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | U266 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | UCHL1 |
| 全名 | ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) |
| 背景介绍 | The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009] |
| 生物功能 | Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. [UniProt] |
| 细胞定位 | Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor. Note=About 30% of total UCHL1 is associated with membranes in brain |
| 预测分子量 | 25 kDa |
| 翻译后修饰 | O-glycosylated. |
检测图片 (3) Click the Picture to Zoom In
-
ARG55814 anti-UCHL1 / PGP9.5 antibody ICC/IF image
Immunofluorescence: A549 cells stained with ARG55814 anti-UCHL1 / PGP9.5 antibody (green) at 1:25 dilution. Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
-
ARG55814 anti-UCHL1 / PGP9.5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney tissue stained with ARG55814 anti-UCHL1 / PGP9.5 antibody at 1:25 dilution.
-
ARG55814 anti-UCHL1 / PGP9.5 antibody WB image
Western blot: 35 µg of U266 cell lysate stained with ARG55814 anti-UCHL1 / PGP9.5 antibody at 1:1000 dilution.
