ARG59243
anti-UBA2 / SAE2 antibody
anti-UBA2 / SAE2 antibody for Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes UBA2 / SAE2 |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | UBA2 / SAE2 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide derived from Human UBA2 / SAE2. |
| 偶联标记 | Un-conjugated |
| 別名 | EC 6.3.2.-; Anthracycline-associated resistance ARX; ARX; SUMO-activating enzyme subunit 2; SAE2; Ubiquitin-like modifier-activating enzyme 2; Ubiquitin-like 1-activating enzyme E1B; HRIHFB2115 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 实际分子量 | ~ 88 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | UBA2 |
| 全名 | ubiquitin-like modifier activating enzyme 2 |
| 背景介绍 | Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010] |
| 生物功能 | The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus. Note=Shuttles between the cytoplasm and the nucleus, sumoylation is required either for nuclear translocation or nuclear retention. [UniProt] |
| 预测分子量 | 71 kDa |
| 翻译后修饰 | Sumoylated with SUMO1 and SUMO2/3 and by UBC9. Sumoylation at Lys-236 inhibits enzymatic activity. Sumoylation at the C-terminal lysine cluster plays an essential role in nuclear trafficking. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG59243 anti-UBA2 / SAE2 antibody WB image
Western blot: A549 cell lysate stained with ARG59243 anti-UBA2 / SAE2 antibody.
