ARG59179
anti-UBA1 / UBE1 antibody
anti-UBA1 / UBE1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes UBA1 / UBE1 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Bov, Rb |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | UBA1 / UBE1 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 1-30 of Human UBA1. |
| 偶联标记 | Un-conjugated |
| 別名 | UBE1; UBE1X; CFAP124; POC20; UBA1A; GXP1; A1S9; A1S9T; Ubiquitin-activating enzyme E1; Ubiquitin-like modifier-activating enzyme 1; Protein A1S9; SMAX2; AMCX1; A1ST |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | K562 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P22314 Human Ubiquitin-like modifier-activating enzyme 1 |
|---|---|
| 基因名称 | UBA1 |
| 全名 | ubiquitin-like modifier activating enzyme 1 |
| 背景介绍 | The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq, Jul 2008] |
| 生物功能 | Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites. [UniProt] |
| 细胞定位 | Cytoplasm. Mitochondrion. Nucleus. Isoform 1: Nucleus. Isoform 2: Cytoplasm. [UniProt] |
| 预测分子量 | 118 kDa |
| 翻译后修饰 | ISGylated. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
-
ARG59179 anti-UBA1 / UBE1 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human testis stained with ARG59179 anti-UBA1 / UBE1 antibody.
-
ARG59179 anti-UBA1 / UBE1 antibody WB image
Western blot: 35 µg of K562 cell lysate stained with ARG59179 anti-UBA1 / UBE1 antibody.
