ARG59835
anti-U2AF1 antibody
anti-U2AF1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes U2AF1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | U2AF1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide within aa. 1-100 of Human U2AF1 (NP_006749.1). |
| 偶联标记 | Un-conjugated |
| 別名 | RN; FP793; U2AF35; U2AFBP; RNU2AF1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Rat spleen, Mouse kidney and 293T | ||||||||
| 实际分子量 | 38 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q01081 Human Splicing factor U2AF 35 kDa subunit Swiss-port # Q9D883 Mouse Splicing factor U2AF 35 kDa subunit |
|---|---|
| 基因名称 | U2AF1 |
| 全名 | U2 small nuclear RNA auxiliary factor 1 |
| 背景介绍 | This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| 生物功能 | Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron. [UniProt] |
| 细胞定位 | Nucleus. Nucleus speckle. [UniProt] |
| 预测分子量 | 28 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG59835 anti-U2AF1 antibody WB image
Western blot: 25 µg of Rat spleen, Mouse kidney and 293T cell lysates stained with ARG59835 anti-U2AF1 antibody at 1:3000 dilution.
