ARG43393
anti-TrpV1 antibody
anti-TrpV1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes TrpV1 |
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| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | TrpV1 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide between aa. 124-153 of Human TrpV1. |
| 偶联标记 | Un-conjugated |
| 別名 | OTRPC1; VR1; Vanilloid receptor 1; Capsaicin receptor; TrpV1; Osm-9-like TRP channel 1; Transient receptor potential cation channel subfamily V member 1 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Mouse cerebellum and Rat cerebellum | ||||||
| 实际分子量 | ~ 95 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TRPV1 |
| 全名 | transient receptor potential cation channel, subfamily V, member 1 |
| 背景介绍 | Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Ligand-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. Involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Activation by vanilloids, like capsaicin, and temperatures higher than 42 degrees Celsius, exhibits a time- and Ca(2+)-dependent outward rectification, followed by a long-lasting refractory state. Mild extracellular acidic pH (6.5) potentiates channel activation by noxious heat and vanilloids, whereas acidic conditions (pH <6) directly activate the channel. Can be activated by endogenous compounds, including 12-hydroperoxytetraenoic acid and bradykinin. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accumbens by affecting AMPA receptors endocytosis. [UniProt] |
| 细胞定位 | Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell projection, dendritic spine membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Note=Mostly, but not exclusively expressed in postsynaptic dendritic spines. [UniProt] |
| 预测分子量 | 95 kDa |
| 翻译后修饰 | Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phosphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PRKCE, PRKCM and probably PRKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG43393 anti-TrpV1 antibody IHC-P image
Immunohistochemistry: Formaldehyde-fixed and paraffin-embedded Human brain tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 9.0). Samples were stained with ARG43393 anti-TrpV1 antibody at 1:500 dilution, for 1 hour at room temperature.
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ARG43393 anti-TrpV1 antibody WB image
Western blot: 20 µg of Mouse cerebellum lysate stained with ARG43393 anti-TrpV1 antibody at 1:2000 dilution.
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ARG43393 anti-TrpV1 antibody WB image
Western blot: 20 µg of Rat cerebellum lysate stained with ARG43393 anti-TrpV1 antibody at 1:2000 dilution.
