ARG59754
anti-Tropomyosin 4 antibody
anti-Tropomyosin 4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Pig
概述
| 产品描述 | Goat Polyclonal antibody recognizes Tropomyosin 4 |
|---|---|
| 反应物种 | Hu, Ms, Pig |
| 预测物种 | Cow, Rat, Dog |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Tropomyosin 4 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the C-terminus of Human Tropomyosin 4. (NP_001138632.1; NP_003281.1. C-ENVGLHQTLDQ) |
| 偶联标记 | Un-conjugated |
| 別名 | HEL-S-108; Tropomyosin-4; Tropomyosin alpha-4 chain; TM30p1 |
应用说明
| 应用建议 |
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| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 32 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TPM4 |
| 全名 | tropomyosin 4 |
| 背景介绍 | This gene encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2009] |
| 生物功能 | Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Binds calcium. [UniProt] |
| 细胞定位 | Cytoplasm, cytoskeleton. Note=Associates with F-actin stress fibers. [UniProt] |
| 预测分子量 | 29 kDa |
检测图片 (5) Click the Picture to Zoom In
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ARG59754 anti-Tropomyosin 4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG59754 anti-Tropomyosin 4 antibody at 2.5 µg/ml dilution followed by AP-staining.
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ARG59754 anti-Tropomyosin 4 antibody WB image
Western blot: 35 µg of Human platelet lysate (in RIPA buffer) stained with ARG59754 anti-Tropomyosin 4 antibody at 0.03 µg/ml dilution and incubated at RT for 1 hour.
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ARG59754 anti-Tropomyosin 4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human prostate tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG59754 anti-Tropomyosin 4 antibody at 2.5 µg/ml dilution followed by AP-staining.
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ARG59754 anti-Tropomyosin 4 antibody WB image
Western blot: 35 µg of NIH/3T3 cell lysate (in RIPA buffer) stained with ARG59754 anti-Tropomyosin 4 antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
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ARG59754 anti-Tropomyosin 4 antibody WB image
Western blot: 35 µg of Pig heart lysate (in RIPA buffer) stained with ARG59754 anti-Tropomyosin 4 antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.
