ARG63731

anti-Triosephosphate isomerase antibody

anti-Triosephosphate isomerase antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Cancer antibody; Metabolism antibody; Signaling Transduction antibody

概述

产品描述 Goat Polyclonal antibody recognizes Triosephosphate isomerase
反应物种 Hu, Ms
预测物种 Cow, Rat, Dog
应用 IHC-P, WB
特异性 This antibody is expected to recognise both reported isoforms.
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 Triosephosphate isomerase
抗原物种 Human
抗原 C-LKPEFVDIINAKQ
偶联标记 Un-conjugated
別名 EC 5.3.1.1; HEL-S-49; TPID; TPI; TIM; Triosephosphate isomerase; Triose-phosphate isomerase

应用说明

应用建议
应用 推荐稀释比
IHC-P2 - 5 µg/ml
WB0.01 - 0.03 µg/ml
应用说明 WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from goat serum by antigen affinity chromatography.
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 21991 Mouse TPI1

GeneID: 7167 Human TPI1

Swiss-port # P17751 Mouse Triosephosphate isomerase

Swiss-port # P60174 Human Triosephosphate isomerase

背景介绍 This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
研究领域 Cancer antibody; Metabolism antibody; Signaling Transduction antibody
预测分子量 31 kDa
翻译后修饰 The initiator methionine for isoform 2 is removed.

检测图片 (3) Click the Picture to Zoom In

  • ARG63731 anti-Triosephosphate isomerase antibody WB image

    Western Blot: human liver lysate (RIPA buffer, 30µg total protein per lane) stained with ARG63731 anti-Triosephosphate isomerase antibody at 0.003 µg/ml dilution.

  • ARG63731 anti-Triosephosphate isomerase antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human pancreas tissue. Antigen Retrieval: Steam tissue section in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG63731 anti-Triosephosphate isomerase antibody at 5 µg/ml dilution followed by AP-staining.

  • ARG63731 anti-Triosephosphate isomerase antibody IHC-P image

    Immunohistochemistry: paraffin embedded Human Liver (Steamed antigen retrieval with Tris/EDTA buffer pH 9) stained with ARG63731 anti-Triosephosphate isomerase antibody at 2 µg/ml dilution followed by HRP-staining.