ARG56967
anti-Tollip antibody [k1B17]
anti-Tollip antibody [k1B17] for ICC/IF,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [k1B17] recognizes Tollip |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | k1B17 |
| 同位型 | IgG2a, kappa |
| 靶点名称 | Tollip |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 61-274 of Human Tollip. |
| 偶联标记 | Un-conjugated |
| 別名 | Toll-interacting protein; IL-1RAcPIP |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TOLLIP |
| 全名 | toll interacting protein |
| 背景介绍 | This gene encodes a ubiquitin-binding protein that interacts with several Toll-like receptor (TLR) signaling cascade components. The encoded protein regulates inflammatory signaling and is involved in interleukin-1 receptor trafficking and in the turnover of IL1R-associated kinase. [provided by RefSeq, Aug 2011] |
| 生物功能 | Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity. Connects the ubiquitin pathway to autophagy by functioning as a ubiquitin-ATG8 family adapter and thus mediating autophagic clearance of ubiquitin conjugates. The TOLLIP-dependent selective autophagy pathway plays an important role in clearance of cytotoxic polyQ proteins aggregates. [UniProt] |
| 预测分子量 | 30 kDa |
| 翻译后修饰 | Phosphorylated by IRAK1 upon stimulation by IL-1 or microbial products. |
检测图片 (2) Click the Picture to Zoom In
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ARG56967 anti-Tollip antibody [k1B17] ICC/IF image
Immunoflorescense: THP-1 cell line stained with ARG56967 anti-Tollip antibody [k1B17] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG56967 anti-Tollip antibody [k1B17] WB image
Western blot: 40 µg of 1) Jurkat cell lysate, 2) HepG2 cell lysate, 3) HeLa cell lysate stained with ARG56967 anti-Tollip antibody [k1B17] at 1:1000.
