ARG57019
anti-Thymine DNA glycosylase antibody [2F7]
anti-Thymine DNA glycosylase antibody [2F7] for Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [2F7] recognizes Thymine DNA glycosylase |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 2F7 |
| 同位型 | IgG1, kappa |
| 靶点名称 | Thymine DNA glycosylase |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-410 of Human Thymine DNA glycosylase. |
| 偶联标记 | Un-conjugated |
| 別名 | G/T mismatch-specific thymine DNA glycosylase; hTDG; Thymine-DNA glycosylase; EC 3.2.2.29 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q13569 Human G/T mismatch-specific thymine DNA glycosylase |
|---|---|
| 基因名称 | TDG |
| 全名 | thymine DNA glycosylase |
| 背景介绍 | The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq, Jul 2008] |
| 生物功能 | DNA glycosylase that plays a key role in active DNA demethylation: specifically recognizes and binds 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) in the context of CpG sites and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine. Cannot remove 5-hydroxymethylcytosine (5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil (5hmU) produced by deamination of 5hmC. Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G/T mispairs to G/C pairs: in the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine. [UniProt] |
| 预测分子量 | 46 kDa |
| 翻译后修饰 | Sumoylation on Lys-330 by either SUMO1 or SUMO2 induces dissociation of the product DNA. |
检测图片 (1) Click the Picture to Zoom In
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ARG57019 anti-Thymine DNA glycosylase antibody [2F7] WB image
Western blot: 30 µg of HeLa cell lysate stained with ARG57019 anti-Thymine DNA glycosylase antibody [2F7] at 1:250.
