ARG55844
anti-Thymidylate Synthase antibody
anti-Thymidylate Synthase antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Thymidylate Synthase |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Thymidylate Synthase |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 265-294 (C-terminus) of Human Thymidylate Synthase. |
| 偶联标记 | Un-conjugated |
| 別名 | Thymidylate synthase; TMS; TSase; TS; HST422; EC 2.1.1.45 |
应用说明
| 应用建议 |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | HL-60 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TYMS |
| 全名 | thymidylate synthetase |
| 背景介绍 | Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase. [provided by RefSeq, Jul 2008] |
| 生物功能 | Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. [UniProt] |
| 细胞定位 | Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane |
| 预测分子量 | 36 kDa |
检测图片 (4) Click the Picture to Zoom In
-
ARG55844 anti-Thymidylate Synthase antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG55844 anti-Thymidylate Synthase antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).
-
ARG55844 anti-Thymidylate Synthase antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human colon carcinoma stained with ARG55844 anti-Thymidylate Synthase antibody.
-
ARG55844 anti-Thymidylate Synthase antibody WB image
Western blot: 35 µg of HL-60 cell lysate stained with ARG55844 anti-Thymidylate Synthase antibody at 1:1000 dilution.
-
ARG55844 anti-Thymidylate Synthase antibody FACS image
Flow Cytometry: WiDr cells stained with ARG55844 anti-Thymidylate Synthase antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC labelled secondary antibody.
