ARG57100
anti-Thymidylate Synthase antibody [21S5]
anti-Thymidylate Synthase antibody [21S5] for Flow cytometry,ICC/IF,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [21S5] recognizes Thymidylate Synthase |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 21S5 |
| 同位型 | IgG2b, kappa |
| 靶点名称 | Thymidylate Synthase |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-313 of Human Thymidylate Synthase |
| 偶联标记 | Un-conjugated |
| 別名 | Thymidylate synthase; TMS; TSase; TS; HST422; EC 2.1.1.45 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TYMS |
| 全名 | thymidylate synthetase |
| 背景介绍 | Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase. [provided by RefSeq, Jul 2008] |
| 生物功能 | Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. [UniProt] |
| 预测分子量 | 36 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG57100 anti-Thymidylate Synthase antibody [21S5] ICC/IF image
Immunofluorescence: HeLa cells line stained with ARG57100 anti-Thymidylate Synthase antibody [21S5] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57100 anti-Thymidylate Synthase antibody [21S5] FACS image
Flow Cytometry: HeLa cell line stained with ARG57100 anti-Thymidylate Synthase antibody [21S5] at 2- 5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).
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ARG57100 anti-Thymidylate Synthase antibody [21S5] WB image
Western blot: 50 ng of 1) Recombinant Human TYMS protein, 40 µg of 2) HeLa, 3) Jurkat, and 4) MCF7 cell lysates stained with ARG57100 anti-Thymidylate Synthase antibody [21S5] at 1:2000.
