ARG40975
anti-Thymidine Kinase antibody
anti-Thymidine Kinase antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Thymidine Kinase |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Thymidine Kinase |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide between aa. 139-173 of Human Thymidine Kinase. |
| 偶联标记 | Un-conjugated |
| 別名 | Thymidine kinase, cytosolic; TK2; EC 2.7.1.21 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | U-2OS | ||||||||
| 实际分子量 | 28 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TK1 |
| 全名 | thymidine kinase 1, soluble |
| 细胞定位 | Cytoplasm. [UniProt] |
| 预测分子量 | 25 kDa |
| 翻译后修饰 | Phosphorylated on Ser-13 in mitosis. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG40975 anti-Thymidine Kinase antibody IHC-P image
Immunohistochemistry: Paraformaldehyde-fixed and paraffin-embedded Human skeletal muscle tissue. Tissue was blocked with 3% BSA for 0.5 hour at room temperature. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). Samples were stained with ARG40975 anti-Thymidine Kinase antibody at 1:25 dilution for 1 hours at 37°C.
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ARG40975 anti-Thymidine Kinase antibody WB image
Western blot: 20 µg of U-2OS cell lysate stained with ARG40975 anti-Thymidine Kinase antibody at 1:2000 dilution.
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ARG40975 anti-Thymidine Kinase antibody FACS image
Flow Cytometry: U2OS cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by ARG40975 anti-Thymidine Kinase antibody (right histogram) at 1:25 dilution for 60 min at 37°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (left histogram) was Rabbit IgG1 (1 µg/10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.
