ARG58012
anti-Thrombomodulin antibody [THBD/1591]
anti-Thrombomodulin antibody [THBD/1591] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [THBD/1591] recognizes Thrombomodulin |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | THBD/1591 |
| 同位型 | IgG2b, kappa |
| 靶点名称 | Thrombomodulin |
| 抗原物种 | Human |
| 抗原 | Recombinant partial protein corresponding to aa. 69-194 of Human Thrombomodulin. |
| 偶联标记 | Un-conjugated |
| 別名 | THRM; CD antigen CD141; THPH12; TM; Thrombomodulin; CD141; BDCA3; Fetomodulin; AHUS6 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Tris buffer with 1 mM EDTA (pH 9.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 0.1 mg/ml BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | THBD |
| 全名 | thrombomodulin |
| 背景介绍 | The protein encoded by this intronless gene is an endothelial-specific type I membrane receptor that binds thrombin. This binding results in the activation of protein C, which degrades clotting factors Va and VIIIa and reduces the amount of thrombin generated. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. [provided by RefSeq, Jul 2008] |
| 生物功能 | Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated. [UniProt] |
| 预测分子量 | 60 kDa (unmodified); 100 kDa (glycosylated) |
| 翻译后修饰 | N-glycosylated. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG58012 anti-Thrombomodulin antibody [THBD/1591] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human cervical carcinoma stained with ARG58012 anti-Thrombomodulin antibody [THBD/1591]. Antigen Retrieval: Boil tissue section in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 10-20 min followed by cooling at RT for 20 min.
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ARG58012 anti-Thrombomodulin antibody [THBD/1591] WB image
Western blot: 1) Recombinant partial protein and 2) THP-1 lysate stained with ARG58012 anti-Thrombomodulin antibody [THBD/1591].
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ARG58012 anti-Thrombomodulin antibody [THBD/1591] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human bladder carcinoma stained with ARG58012 anti-Thrombomodulin antibody [THBD/1591]. Antigen Retrieval: Boil tissue section in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 10-20 min followed by cooling at RT for 20 min.
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ARG58012 anti-Thrombomodulin antibody [THBD/1591] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human placenta stained with ARG58012 anti-Thrombomodulin antibody [THBD/1591]. Antigen Retrieval: Boil tissue section in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 10-20 min followed by cooling at RT for 20 min.
