anti-TdT antibody [SQab20183]
anti-TdT antibody [SQab20183] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
|产品描述||Recombinant Rabbit Monoclonal antibody [SQab20183] recognizes TdT|
|抗原||Synthetic peptide within aa. 1-100 of Human TdT.|
|別名||EC 188.8.131.52; Terminal addition enzyme; Terminal transferase; DNA nucleotidylexotransferase; Terminal deoxynucleotidyltransferase; TDT|
|应用说明||IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|纯化||Purification with Protein A.|
|缓冲液||PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.|
|抗菌剂||0.01% Sodium azide|
|稳定剂||40% Glycerol and 0.05% BSA|
|存放说明||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|注意事项||For laboratory research only, not for drug, diagnostic or other use.|
|背景介绍||This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]|
|生物功能||Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells. [UniProt]|
检测图片 (2) Click the Picture to Zoom In
ARG66750 anti-TdT antibody [SQab20183] WB image (Customer's Feedback)
Western blot: JurKat and Molt-4 cell lysate stained with ARG66750 anti-TdT antibody [SQab20183] at 1:1000 dilution.
ARG66750 anti-TdT antibody [SQab20183] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human thymoma tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66750 anti-TdT antibody [SQab20183] at 18°C - 25°C for 30 minutes.