ARG45433

anti-TSEN34 antibody

anti-TSEN34 antibody for ICC/IF,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes TSEN34
反应物种 Hu, Ms, Rat
应用 ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 TSEN34
抗原物种 Human
抗原 Recombinant protein containing to human TSEN34.
偶联标记 Un-conjugated
別名 TSEN34; TRNA Splicing Endonuclease Subunit 34; SEN34; SEN34L; LENG5; Leukocyte Receptor Cluster (LRC) Member 5; TSEN34 TRNA Splicing Endonuclease Subunit; TRNA-Splicing Endonuclease Subunit Sen34; Leukocyte Receptor Cluster Member 5; TRNA-Intron Endonuclease Sen34; TRNA Splicing Endonuclease 34 Homolog (SEN34, S. Cerevisiae); TRNA Splicing Endonuclease 34 Homolog (S. Cerevisiae); CTD-3093M3.1; EC 4.6.1.16; HsSen34; PCH2C

应用说明

应用建议
应用 推荐稀释比
ICC/IF5 μg/ml
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 50 kDa

属性

形式 Liquid
纯化 Affinity purified
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 66078 Mouse TSEN34

GeneID: 79042 Human TSEN34

Swiss-port # Q8BMZ5 Mouse tRNA-splicing endonuclease subunit Sen34

Swiss-port # Q9BSV6 Human tRNA-splicing endonuclease subunit Sen34

基因名称 TSEN34
全名 TRNA Splicing Endonuclease Subunit
背景介绍 This gene encodes a catalytic subunit of the tRNA splicing endonuclease, which catalyzes the removal of introns from precursor tRNAs. The endonuclease complex is also associated with a pre-mRNA 3-prime end processing factor. A mutation in this gene results in the neurological disorder pontocerebellar hypoplasia type 2. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Oct 2009]
生物功能 Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. [UniProt]
细胞定位 Nucleus. [UniProt]
预测分子量 34 kDa

检测图片 (3) Click the Picture to Zoom In

  • ARG45433 anti-TSEN34 antibody ICC/IF image

    Immunofluorescence: U87 stained with ARG45433 anti-TSEN34 antibody at 5 μg/ml dilution.

  • ARG45433 anti-TSEN34 antibody WB image

    Western blot: Rat brain stained with ARG45433 anti-TSEN34 antibody at 0.5 μg/ml dilution.

  • ARG45433 anti-TSEN34 antibody WB image

    Western blot: Mouse brain stained with ARG45433 anti-TSEN34 antibody at 0.5 μg/ml dilution.