ARG22280
anti-TRPM7 / LTRPC7 antibody [S74-25]
anti-TRPM7 / LTRPC7 antibody [S74-25] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Mouse Monoclonal antibody [S74-25] recognizes TRPM7 / LTRPC7 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 特异性 | Detects ~220kDa. No cross-reactivity against TrpM6. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | S74-25 |
| 同位型 | IgG1 |
| 靶点名称 | TRPM7 / LTRPC7 |
| 抗原物种 | Mouse |
| 抗原 | Fusion protein around aa. 1817-1863 (C- terminus) of Mouse TrpM7 |
| 偶联标记 | Un-conjugated |
| 別名 | Transient receptor potential cation channel subfamily M member 7; CHAK; ALSPDC; LTrpC7; LTRPC7; CHAK1; Channel-kinase 1; TRP-PLIK; EC 2.7.11.1; LTrpC-7; Long transient receptor potential channel 7 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q923J1 Mouse Transient receptor potential cation channel subfamily M member 7 Swiss-port # Q96QT4 Human Transient receptor potential cation channel subfamily M member 7 |
|---|---|
| 基因名称 | Trpm7 |
| 全名 | transient receptor potential cation channel, subfamily M, member 7 |
| 背景介绍 | The protein encoded by this gene is both an ion channel and a serine/threonine protein kinase. The kinase activity is essential for the ion channel function, which serves to increase intracellular calcium levels and to help regulate magnesium ion homeostasis. Defects in this gene are a cause of amyotrophic lateral sclerosis-parkinsonism/dementia complex of Guam. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| 生物功能 | Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. Involved in TNF-induced necroptosis downstream of MLKL by mediating calcium influx. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1). [UniProt] |
| 细胞定位 | Membrane |
| 预测分子量 | 213 kDa |
| 翻译后修饰 | Autophosphorylated. |
检测图片 (2) Click the Picture to Zoom In
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ARG22280 anti-TRPM7 / LTRPC7 antibody [S74-25] IHC image
Immunohistochemistry: 10% Formalin (12-24 hours at RT) fixed Mouse Brain slice stained with ARG22280 anti-TRPM7 / LTRPC7 antibody [S74-25] (brown) at 1:1000 dilution (1 hour). Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500uL for 5 minutes at RT.
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ARG22280 anti-TRPM7 / LTRPC7 antibody [S74-25] WB image
Western blot: Human cell lysates stained with ARG22280 anti-TRPM7 / LTRPC7 antibody [S74-25] at 1:1000 dilution.
