ARG40778
anti-TRPM2 antibody
anti-TRPM2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes TRPM2 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow, Dog, Gpig, Hrs |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | TRPM2 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the N-terminal region of Human TRPM2. (Within the following region: VAILQALLKASRSQDHFGHENWDHQLKLAVAWNRVDIARSEIFMDEWQWK) |
| 偶联标记 | Un-conjugated |
| 別名 | KNP3; Transient receptor potential cation channel subfamily M member 2; NUDT9H; EREG1; LTrpC2; TrpC7; EC 3.6.1.13; TRPC7; NUDT9L1; LTRPC2; LTrpC-2; Transient receptor potential channel 7; Long transient receptor potential channel 2; Estrogen-responsive element-associated gene 1 protein |
应用说明
| 预测反应性说明 | Predicted Homology Based On Immunogen Sequence: Cow: 93%; Dog: 86%; Guinea pig: 88%; Horse: 93%; Mouse: 100%; Rat: 100% | ||||||
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| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS, 0.09% (w/v) Sodium azide and 2% Sucrose. |
| 抗菌剂 | 0.09% (w/v) Sodium azide |
| 稳定剂 | 2% Sucrose |
| 浓度 | Batch dependent: 0.5 - 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O94759 Human Transient receptor potential cation channel subfamily M member 2 |
|---|---|
| 基因名称 | TRPM2 |
| 全名 | transient receptor potential cation channel, subfamily M, member 2 |
| 背景介绍 | The protein encoded by this gene is a calcium-permeable cation channel that is regulated by free intracellular ADP-ribose. The encoded protein is activated by oxidative stress and confers susceptibility to cell death. Several alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2008] |
| 生物功能 | Nonselective, voltage-independent cation channel mediating sodium and calcium ion influx in response to oxidative stress. Extracellular calcium passes through the channel and acts from the intracellular side as a positive regulator in channel activation. Activated by ADP-ribose, nicotinamide adenine dinucleotide (NAD(+)), reactive nitrogen species and arachidonic acid. Inactivated by intracellular ATP. Confers susceptibility to cell death following oxidative stress. Isoform 2 does not seem to be regulated by ADPR. Has ADP-ribose pyrophosphatase activity. [UniProt] |
| 细胞定位 | Isoform 1,2 and 3: Cell membrane; Multi-pass membrane protein. Note=Detected at the cell membrane and in intracellular vesicles in cortical neurons. Detected on neuronal cell bodies and neurites. Detected on the cell membrane in polymorphonuclear neutrophils. Detected on cytoplasmic vesicles and lysosomes in immature bone marrow dendritic cells. [UniProt] |
| 预测分子量 | 171 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG40778 anti-TRPM2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human heart tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40778 anti-TRPM2 antibody at 10 µg/ml, overnight at 4°C.
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ARG40778 anti-TRPM2 antibody WB image
Western blot: Human fetal liver lysate stained with ARG40778 anti-TRPM2 antibody at 1 µg/ml dilution.
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ARG40778 anti-TRPM2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human brain tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40778 anti-TRPM2 antibody at 10 µg/ml, overnight at 4°C.
