ARG22234
anti-TRPC5 antibody [S67-15]
anti-TRPC5 antibody [S67-15] for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Mouse Monoclonal antibody [S67-15] recognizes TRPC5 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, IP, WB |
| 特异性 | Detects ~110kDa. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | S67-15 |
| 同位型 | IgG2b |
| 靶点名称 | TRPC5 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around aa. 827-845 of Human TrpC5 |
| 偶联标记 | Un-conjugated |
| 別名 | hTRP-5; hTRP5; PPP1R159; Transient receptor protein 5; TrpC5; TRP-5; Short transient receptor potential channel 5; TRP5 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9QX29 Mouse Short transient receptor potential channel 5 Swiss-port # Q9UL62 Human Short transient receptor potential channel 5 |
|---|---|
| 基因名称 | TRPC5 |
| 全名 | transient receptor potential cation channel, subfamily C, member 5 |
| 背景介绍 | This gene belongs to the transient receptor family. It encodes one of the seven mammalian TRPC (transient receptor potential channel) proteins. The encoded protein is a multi-pass membrane protein and is thought to form a receptor-activated non-selective calcium permeant cation channel. The protein is active alone or as a heteromultimeric assembly with TRPC1, TRPC3, and TRPC4. It also interacts with multiple proteins including calmodulin, CABP1, enkurin, Na(+)-H+ exchange regulatory factor (NHERF ), interferon-induced GTP-binding protein (MX1), ring finger protein 24 (RNF24), and SEC14 domain and spectrin repeat-containing protein 1 (SESTD1). [provided by RefSeq, May 2010] |
| 生物功能 | Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Has also been shown to be calcium-selective (By similarity). May also be activated by intracellular calcium store depletion. [UniProt] |
| 细胞定位 | Membrane |
| 预测分子量 | 111 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG22234 anti-TRPC5 antibody [S67-15] IHC image
Immunohistochemistry: 10% Formalin (12-24 hours at RT) fixed Mouse Brain slice stained with ARG22234 anti-TRPC5 antibody [S67-15] (brown) at 1:1000 dilution (1 hour). Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500uL for 5 minutes at RT.
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ARG22234 anti-TRPC5 antibody [S67-15] WB image
Western blot: 20 µg of Rat Brain Membrane cell lysate. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary antibody: ARG22234 anti-TRPC5 antibody [S67-15] at 1:1000 for 16 hours at 4°C. Secondary antibody: Goat anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT.
