ARG23866
anti-TROP2 antibody [M005]
anti-TROP2 antibody [M005] for ELISA,ICC/IF,Immunoprecipitation,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [M005] recognizes TROP2 |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA, ICC/IF, IP, WB |
| 特异性 | This antibody detects a 50-65 kDa band corresponding to the molecular mass of TROP2 on SDS-PAGE immunoblots of native human A431, MDA-MB-231, and MCF-7 cell lysates. The antibody also detects denatured TROP2, but with less affinity. Lower dilutions of the antibody should be used for denatured western blot. Clone M005 can be used for western blot, immunocytochemistry, ELISA, and immunoprecipitation, as well as for detecting live unfixed cells. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | M005 |
| 同位型 | IgG1 |
| 靶点名称 | TROP2 |
| 抗原物种 | Human |
| 抗原 | Extracellular region of Human TROP2 from the A431 epidermoid carcinoma cell line. |
| 偶联标记 | Un-conjugated |
| 別名 | Membrane component chromosome 1 surface marker 1; M1S1; Cell surface glycoprotein Trop-2; TROP2; EGP-1; EGP1; Tumor-associated calcium signal transducer 2; GA733-1; Pancreatic carcinoma marker protein GA733-1; GA7331; GP50 |
应用说明
| 应用建议 |
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| 应用说明 | WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | 50 - 65 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 50% Glycerol and 1 mg/ml BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P09758 Human Tumor-associated calcium signal transducer 2 |
|---|---|
| 基因名称 | TACSTD2 |
| 全名 | tumor-associated calcium signal transducer 2 |
| 背景介绍 | This intronless gene encodes a carcinoma-associated antigen. This antigen is a cell surface receptor that transduces calcium signals. Mutations of this gene have been associated with gelatinous drop-like corneal dystrophy.[provided by RefSeq, Dec 2009] |
| 生物功能 | May function as a growth factor receptor. [UniProt] |
| 预测分子量 | 36 kDa |
| 翻译后修饰 | The N-terminus is blocked. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG23866 anti-TROP2 antibody [M005] ICC/IF image
Immunofluorescence: Paraformaldehyde-fixed A431 cells stained with ARG23866 anti-TROP2 antibody [M005] (red).
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ARG23866 anti-TROP2 antibody [M005] WB image
Western blot: A431, MDA-MB-231 and MCF-7 cell lysates under native ("n") and denatured ("d") conditions. The blots were stained with ARG23866 anti-TROP2 antibody [M005] at 1:1000 dilution.
