ARG65217
anti-TRIM71 antibody
anti-TRIM71 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes TRIM71 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Cow, Rat, Dog |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | TRIM71 |
| 抗原物种 | Human |
| 抗原 | C-RQVKAKSLYLQVE |
| 偶联标记 | Un-conjugated |
| 別名 | EC 6.3.2.-; Tripartite motif-containing protein 71; Protein lin-41 homolog; LIN-41; LIN41; E3 ubiquitin-protein ligase TRIM71 |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q1PSW8 Mouse E3 ubiquitin-protein ligase TRIM71 Swiss-port # Q2Q1W2 Human E3 ubiquitin-protein ligase TRIM71 |
|---|---|
| 基因名称 | TRIM71 |
| 全名 | tripartite motif containing 71, E3 ubiquitin protein ligase |
| 生物功能 | E3 ubiquitin-protein ligase that cooperates with the microRNAs (miRNAs) machinery and promotes embryonic stem cells proliferation and maintenance. Binds to miRNAs and associates with AGO2, participating in post-transcriptional repression of transcripts such as CDKN1A. Facilitates the G1-S transition to promote rapid embryonic stem cell self-renewal by repressing CDKN1A expression. Required to maintain proliferation and prevent premature differentiation of neural progenitor cells during early neural development: positively regulates FGF signaling by controlling the stability of SHCBP1 (By similarity). [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody |
| 预测分子量 | 93 kDa |
| 翻译后修饰 | Autoubiquitinated. |
检测图片 (2) Click the Picture to Zoom In
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ARG65217 anti-TRIM71 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cerebellum tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65217 anti-TRIM71 antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG65217 anti-TRIM71 antibody WB image
Western blot: 35 µg of HEK293 (A) and NIH/3T3 (B) cell lysates (in RIPA buffer) stained with ARG65217 anti-TRIM71 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
