ARG54665
anti-TRIM71 antibody
anti-TRIM71 antibody for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes TRIM71 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ELISA, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | TRIM71 |
| 抗原 | Synthetic peptide (15 aa) within aa. 680-730 of Human TRIM71. |
| 偶联标记 | Un-conjugated |
| 別名 | EC 6.3.2.-; Tripartite motif-containing protein 71; Protein lin-41 homolog; LIN-41; LIN41; E3 ubiquitin-protein ligase TRIM71 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | Human Brain Tissue Lysate |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TRIM71 |
| 全名 | tripartite motif containing 71, E3 ubiquitin protein ligase |
| 背景介绍 | TRIM71 Antibody: TRIM71 is the mammalian ortholog to the C. elegans heterochronic protein Lin41, a protein that is thought to be important in postembryonic development, and is genetically and biochemically downstream of both Shh and Fgf signaling pathways. Similar to its C. elegans homolog, TRIM71 is a target of the microRNA let-7 and is likely to play a role in mammalian development. Recent experiments have indicated that TRIM71 is an E3 ubiquitin ligase and can interact with the Dicer and the Argonaute proteins Ago1, Ago2, and Ago4. Overexpression and depletion of TRIM71 led to inverse changes in Ago2 protein levels, suggesting TRIM71 can regulate Ago2 turnover. Finally, TRIM71 cooperates with the pluripotency factor Lin-28 in regulating let-7. | |
| 研究领域 | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody |
| 预测分子量 | 93 kDa |
| 翻译后修饰 | Autoubiquitinated. |
检测图片 (3) Click the Picture to Zoom In
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ARG54665 anti-TRIM71 antibody ICC/IF image
Immunofluorescence: human brain cells stained with ARG54665 anti-TRIM71 antibody at 20 μg/ml.
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ARG54665 anti-TRIM71 antibody IHC image
Immunohistochemistry: human brain tissue stained with ARG54665 anti-TRIM71 antibody at 5 μg/ml.
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ARG54665 anti-TRIM71 antibody WB image
Western blot: human brain tissue lysate stained with ARG54665 anti-TRIM71 antibody at (A) 1 and (B) 2 μg/ml.
