ARG40770
anti-TRIM63 / MuRF1 antibody
anti-TRIM63 / MuRF1 antibody for ICC/IF,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes TRIM63 / MuRF1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 预测物种 | Pig |
| 应用 | ICC/IF, WB |
| 特异性 | This antibody detects 38 and 30 kDa proteins corresponding to the apparent molecular mass of MuRF1 isoforms on SDS-PAGE immunoblots of C2C12 cells, and detects a ~ 38 kDa band in mouse heart and muscle tissue. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | TRIM63 / MuRF1 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide around the C-terminus of Human MuRF1. |
| 偶联标记 | Un-conjugated |
| 別名 | E3 ubiquitin-protein ligase TRIM63; Muscle-specific RING finger protein 1; EC 6.3.2.-; MURF2; MURF1; MuRF-1; Striated muscle RING zinc finger protein; SMRZ; RNF28; RING finger protein 28; Tripartite motif-containing protein 63; MuRF1; IRF; Iris RING finger protein |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||
| 实际分子量 | 38 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 50% Glycerol and 1 mg/ml BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q91Z63 Rat E3 ubiquitin-protein ligase TRIM63 Swiss-port # Q969Q1 Human E3 ubiquitin-protein ligase TRIM63 |
|---|---|
| 基因名称 | TRIM63 |
| 全名 | tripartite motif containing 63, E3 ubiquitin protein ligase |
| 背景介绍 | This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is an E3 ubiquitin ligase that localizes to the Z-line and M-line lattices of myofibrils. This protein plays an important role in the atrophy of skeletal and cardiac muscle and is required for the degradation of myosin heavy chain proteins, myosin light chain, myosin binding protein, and for muscle-type creatine kinase. [provided by RefSeq, Feb 2012] |
| 生物功能 | E3 ubiquitin ligase. Mediates the ubiquitination and subsequent proteasomal degradation of CKM, GMEB1 and HIBADH. Regulates the proteasomal degradation of muscle proteins under amino acid starvation, where muscle protein is catabolized to provide other organs with amino acids. Inhibits de novo skeletal muscle protein synthesis under amino acid starvation. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells. [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus. Cytoplasm, myofibril, sarcomere, M line. Cytoplasm, myofibril, sarcomere, Z line. Note=Colocalizes with TNNI3 in myocytes (By similarity). Localizes to the M- and Z-lines in skeletal muscle. [UniProt] |
| 预测分子量 | 40 kDa |
检测图片 (2) Click the Picture to Zoom In
-
ARG40770 anti-TRIM63 / MuRF1 antibody ICC/IF image
Immunofluorescence: C2C12 cells were stained with ARG40770 anti-TRIM63 / MuRF1 antibody (red), in the absence (left) or presence (right) of blocking peptide.
-
ARG40770 anti-TRIM63 / MuRF1 antibody WB image
Western blot: Mouse heart and C2C12 cell lysates stained with ARG40770 anti-TRIM63 / MuRF1 antibody.
