ARG63496
anti-TRIM37 antibody
anti-TRIM37 antibody for Flow cytometry,ICC/IF and Human
概述
| 产品描述 | Goat Polyclonal antibody recognizes TRIM37 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Cow, Dog |
| 应用 | FACS, ICC/IF |
| 特异性 | Reported variants represent identical protein (NP_056109.1; NP_001005207.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | TRIM37 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the C-terminus of Human TRIM37. (C-EDLSFNTDENSGR) (NP_056109.1) |
| 偶联标记 | Un-conjugated |
| 別名 | TRIM37; tripartite motif-containing 37; MUL; TEF3; KIAA0898; MUL protein; RING-B-box-coiled-coil protein; POB1; tripartite motif-containing 37 protein |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O94972 Human E3 ubiquitin-protein ligase TRIM37 |
|---|---|
| 基因名称 | TRIM37 |
| 全名 | tripartite motif containing 37 |
| 背景介绍 | This gene encodes a member of the tripartite motif (TRIM) family, whose members are involved in diverse cellular functions such as developmental patterning and oncogenesis. The TRIM motif includes zinc-binding domains, a RING finger region, a B-box motif and a coiled-coil domain. The RING finger and B-box domains chelate zinc and might be involved in protein-protein and/or protein-nucleic acid interactions. The gene mutations are associated with mulibrey (muscle-liver-brain-eye) nanism, an autosomal recessive disorder that involves several tissues of mesodermal origin. [provided by RefSeq, Mar 2016] |
| 生物功能 | E3 ubiquitin-protein ligase required to prevent centriole reduplication (PubMed:15885686, PubMed:23769972). Probably acts by ubiquitinating positive regulators of centriole reduplication (PubMed:23769972). Mediates monoubiquitination of 'Lys-119' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression: associates with some Polycomb group (PcG) multiprotein PRC2-like complex and mediates repression of target genes (PubMed:25470042). Has anti-HIV activity (PubMed:24317724). [UniProt] |
| 细胞定位 | Cytoplasm, perinuclear region. Peroxisome. Note=Found in vesicles of the peroxisome. Aggregates as aggresomes, a perinuclear region where certain misfolded or aggregated proteins are sequestered for proteasomal degradation. [UniProt] |
| 预测分子量 | 108 kDa |
| 翻译后修饰 | Auto-ubiquitinated. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG63496 anti-TRIM37 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde-fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG63496 anti-TRIM37 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized Goat IgG (green) at 10 µg/ml dilution.
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ARG63496 anti-TRIM37 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HeLa cells permeabilized with 0.5% Triton. Cells were stained with ARG63496 anti-TRIM37 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa Fluor® 488 labelled secondary antibody. IgG control: Unimmunized Goat IgG (black line) followed by Alexa Fluor® 488 labelled secondary antibody.
