ARG40744
anti-TRIM25 antibody
anti-TRIM25 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes TRIM25 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | TRIM25 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide derived from Human TRIM25. |
| 偶联标记 | Un-conjugated |
| 別名 | EC 6.3.2.19; ZNF147; EFP; Ubiquitin/ISG15-conjugating enzyme TRIM25; Tripartite motif-containing protein 25; Z147; E3 ubiquitin/ISG15 ligase TRIM25; EC 6.3.2.n3; Zinc finger protein 147; Estrogen-responsive finger protein; RNF147; RING finger protein 147 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TRIM25 |
| 全名 | tripartite motif containing 25 |
| 背景介绍 | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. [UniProt] |
| 细胞定位 | Cytoplasm. Note=Colocalized with DDX58 at cytoplasmic perinuclear bodies. [UniProt] |
| 预测分子量 | 71 kDa |
| 翻译后修饰 | Auto-ISGylated. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG40744 anti-TRIM25 antibody WB image
Western blot: HeLa cell lysate stained with ARG40744 anti-TRIM25 antibody.
