ARG63260
anti-TRIM2 antibody
anti-TRIM2 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
Cell Biology and Cellular Response antibody; Gene Regulation antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes TRIM2 |
|---|---|
| 反应物种 | Hu, Rat |
| 预测物种 | Ms, Cow, Dog |
| 应用 | FACS, IHC-P, WB |
| 特异性 | This antibody is expected to recognise both reported isoforms (NP_056086.2; NP_001123539.1). Cross-reactivity with TRIM3 has been demonstrated. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | TRIM2 |
| 抗原物种 | Human |
| 抗原 | DSGNHCFKVYRYLQ |
| 偶联标记 | Un-conjugated |
| 別名 | RNF86; EC 6.3.2.-; RING finger protein 86; Tripartite motif-containing protein 2; CMT2R; E3 ubiquitin-protein ligase TRIM2 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # D3ZQG6 Rat Tripartite motif-containing protein 2 Swiss-port # Q9C040 Human Tripartite motif-containing protein 2 |
|---|---|
| 背景介绍 | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic filaments. Its function has not been identified. [provided by RefSeq, Jul 2008] |
| 研究领域 | Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| 预测分子量 | 82 kDa |
| 翻译后修饰 | RING-type zinc finger-dependent and UBE2D1-dependent autoubiquitination. |
检测图片 (3) Click the Picture to Zoom In
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ARG63260 anti-TRIM2 antibody WB image
Western Blot: Rat Brain lysate (35 µg protein in RIPA buffer) stained with ARG63260 anti-TRIM2 antibody at 0.5 µg/ml dilution.
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ARG63260 anti-TRIM2 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Cortex. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63260 anti-TRIM2 antibody at 6.3 µg/ml dilution followed by AP-staining.
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ARG63260 anti-TRIM2 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed Jurkat cells permeabilized with 0.5% Triton. Cells were stained with ARG63260 anti-TRIM2 antibody (blue line) at 10 µg/ml dilution and overnight, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
