ARG42062
anti-TREM2 antibody [RM0139-5J46]
anti-TREM2 antibody [RM0139-5J46] for ICC/IF,IHC-Frozen sections,Western blot and Mouse
概述
| 产品描述 | Rat Monoclonal antibody [RM0139-5J46] recognizes TREM2 |
|---|---|
| 反应物种 | Ms |
| 应用 | ICC/IF, IHC-Fr, WB |
| 特异性 | This antibody detects Mouse of TREM2, but not TREM1. |
| 宿主 | Rat |
| 克隆 | Monoclonal |
| 克隆号 | RM0139-5J46 |
| 同位型 | IgG2 |
| 靶点名称 | TREM2 |
| 抗原物种 | Mouse |
| 抗原 | The extracellular domain of Mouse TREM2. |
| 偶联标记 | Un-conjugated |
| 別名 | Trem2a; Trem2b; TREM-2; Triggering receptor expressed on myeloid cells 2; Triggering receptor expressed on monocytes 2; Trem2c |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Mouse microglia cells |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A/G. |
| 纯化说明 | 0.2 µm filter sterilized. |
| 缓冲液 | PBS |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q99NH8 Mouse Triggering receptor expressed on myeloid cells 2 |
|---|---|
| 基因名称 | TREM2 |
| 全名 | triggering receptor expressed on myeloid cells 2 |
| 背景介绍 | This gene encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012] |
| 生物功能 | Forms a receptor signaling complex with TYROBP which mediates signaling and cell activation following ligand binding (PubMed:10799849). Acts as a receptor for amyloid-beta protein 42, a cleavage product of the amyloid-beta precursor protein APP, and mediates its uptake and degradation by microglia (PubMed:27477018, PubMed:29518356). Binding to amyloid-beta 42 mediates microglial activation, proliferation, migration, apoptosis and expression of pro-inflammatory cytokines, such as IL6R and CCL3, and the anti-inflammatory cytokine ARG1 (By similarity). Acts as a receptor for lipoprotein particles such as LDL, VLDL, and HDL and for apolipoproteins such as APOA1, APOA2, APOB, APOE, APOE2, APOE3, APOE4, and CLU and enhances their uptake in microglia (PubMed:27477018). Binds phospholipids (preferably anionic lipids) such as phosphatidylserine, phosphatidylethanolamine, phosphatidylglycerol and sphingomyelin (PubMed:29794134). Regulates microglial proliferation by acting as an upstream regulator of the Wnt/beta-catenin signaling cascade (By similarity). Required for microglial phagocytosis of apoptotic neurons (PubMed:24990881). Also required for microglial activation and phagocytosis of myelin debris after neuronal injury and of neuronal synapses during synapse elimination in the developing brain (By similarity). Regulates microglial chemotaxis and process outgrowth, and also the microglial response to oxidative stress and lipopolysaccharide (By similarity). It suppresses PI3K and NF-kappa-B signaling in response to lipopolysaccharide; thus promoting phagocytosis, suppressing pro-inflammatory cytokine and nitric oxide production, inhibiting apoptosis and increasing expression of IL10 and TGFB (By similarity). During oxidative stress, it promotes anti-apoptotic NF-kappa-B signaling and ERK signaling (By similarity). Plays a role in microglial MTOR activation and metabolism (By similarity). Regulates age-related changes in microglial numbers (PubMed:29752066). Triggers activation of the immune responses in macrophages and dendritic cells (PubMed:10799849). Mediates cytokine-induced formation of multinucleated giant cells which are formed by the fusion of macrophages (By similarity). In dendritic cells, it mediates up-regulation of chemokine receptor CCR7 and dendritic cell maturation and survival (PubMed:11602640). Involved in the positive regulation of osteoclast differentiation (PubMed:12925681). [UniProt] |
| 细胞定位 | Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Isoform 3: Secreted. [UniProt] |
| 预测分子量 | 25 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG42062 anti-TREM2 antibody [RM0139-5J46] ICC/IF image
Immunofluorescence: Mouse microglia cells stained with ARG42062 anti-TREM2 antibody [RM0139-5J46].
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ARG42062 anti-TREM2 antibody [RM0139-5J46] WB image
Western blot: Mouse microglia cells untreated or treated with TREM2-siRNA. Cell lysates were stained with ARG42062 anti-TREM2 antibody [RM0139-5J46].
