ARG23275
anti-TREM1 antibody [L5-B8.2A12.3A12] (low endotoxin)
anti-TREM1 antibody [L5-B8.2A12.3A12] (low endotoxin) for Flow cytometry,Functional study,ICC/IF,IHC-Frozen sections,Western blot and Mouse
概述
| 产品描述 | Azide free and low endotoxin Rat Monoclonal antibody [L5-B8.2A12.3A12] recognizes TREM1 Rat anti Mouse Trem-1 antibody, clone L5-B8. 2A12. 3A12 recognizes mouse triggering receptor expressed on myeloid cells 1 (TREM-1), a 30kD member of the immunoglobulin superfamily expressed on neutrophils and a subset of monocytes. TREM-1 is involved in the microbial inflammatory response. It acts in conjunction with the adaptor protein DAP12 to activate neutrophils and monocytes, leading to the secretion of pro-inflammatory mediators and enhancement of the inflammatory response. Microbial infection also induces the release of soluble form of TREM-1 from monocytes, which is reported to act as an inhibitor of TREM-1. TREM-1 mediates the septic shock pathway, and blocking TREM-1 has been reported to reduce inflammation and increase survival in certain models of bacterial infection. |
|---|---|
| 反应物种 | Ms |
| 应用 | FACS, FuncSt, ICC/IF, IHC-Fr, WB |
| 宿主 | Rat |
| 克隆 | Monoclonal |
| 克隆号 | L5-B8.2A12.3A12 |
| 同位型 | IgG2a |
| 靶点名称 | TREM1 |
| 抗原物种 | Mouse |
| 抗原 | Mouse TREM-1 Fc fusion protein. |
| 偶联标记 | Un-conjugated |
| 別名 | TREM-1; CD antigen CD354; CD354; Triggering receptor expressed on monocytes 1; Triggering receptor expressed on myeloid cells 1 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-Fr: The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Arigo recommends the use of acetone fixation for frozen sections. FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 纯化说明 | Low endotoxin |
| 缓冲液 | PBS. |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9JKE2 Mouse Triggering receptor expressed on myeloid cells 1 |
|---|---|
| 基因名称 | TREM1 |
| 全名 | triggering receptor expressed on myeloid cells 1 |
| 背景介绍 | This gene encodes a receptor belonging to the Ig superfamily that is expressed on myeloid cells. This protein amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011] |
| 生物功能 | Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock. [UniProt] |
| 预测分子量 | 26 kDa |
| 翻译后修饰 | Glycosylated. [UniProt] |
