ARG64891
anti-TRC8 antibody
anti-TRC8 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes TRC8 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Dog |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | TRC8 |
| 抗原物种 | Human |
| 抗原 | C-HTGAAAEEFNDDT |
| 偶联标记 | Un-conjugated |
| 別名 | EC 6.3.2.-; TRC8; RING finger protein 139; RCA1; E3 ubiquitin-protein ligase RNF139; HRCA1; Translocation in renal carcinoma on chromosome 8 protein |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q8WU17 Human E3 ubiquitin-protein ligase RNF139 |
|---|---|
| 背景介绍 | The protein encoded by this gene is a multi-membrane spanning protein containing a RING-H2 finger. This protein is located in the endoplasmic reticulum, and has been shown to possess ubiquitin ligase activity. This gene was found to be interrupted by a t(3:8) translocation in a family with hereditary renal and non-medulary thyroid cancer. Studies of the Drosophila counterpart suggested that this protein may interact with tumor suppressor protein VHL, as well as with COPS5/JAB1, a protein responsible for the degradation of tumor suppressor CDKN1B/P27KIP. [provided by RefSeq, Jul 2008] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody |
| 预测分子量 | 76 kDa |
| 翻译后修饰 | Autoubiquitinated. Ubiquitination is induced by sterol and leads to ist degradation via the ubiquitin-proteasome pathway. |
检测图片 (2) Click the Picture to Zoom In
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ARG64891 anti-TRC8 antibody WB image
Western Blot: Human Skeletal Muscle lysate (35 µg protein in RIPA buffer) stained with ARG64891 anti-TRC8 antibody at 1 µg/ml dilution.
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ARG64891 anti-TRC8 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Placenta. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64891 anti-TRC8 antibody at 3.8 µg/ml dilution followed by AP-staining.
