ARG65293
anti-TPPP / p25 antibody
anti-TPPP / p25 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes TPPP / p25 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | TPPP / p25 |
| 抗原物种 | Human |
| 抗原 | C-DPSKDRAAKRLSLES |
| 偶联标记 | Un-conjugated |
| 別名 | TPPP1; TPPP; p25-alpha; p25; p24; Tubulin polymerization-promoting protein; 25 kDa brain-specific protein; p25alpha; TPPP/p25 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Microwaved or steamed tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O94811 Human Tubulin polymerization-promoting protein Swiss-port # Q7TQD2 Mouse Tubulin polymerization-promoting protein |
|---|---|
| 基因名称 | TPPP |
| 全名 | tubulin polymerization promoting protein |
| 生物功能 | May play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. May play a role in mitotic spindle assembly and nuclear envelope breakdown. [UniProt] |
| 研究领域 | Signaling Transduction antibody |
| 预测分子量 | 24 kDa |
| 翻译后修饰 | Poor substrate for GSK3 (By similarity). Phosphorylated by LIMK1 on serine residues. Phosphorylation may alter the tubulin polymerization activity. |
检测图片 (5) Click the Picture to Zoom In
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ARG65293 anti-TPPP / p25 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cerebral cortex (steamed antigen retrieval with citrate buffer pH 6.0) stained with ARG65293 anti-TPPP / p25 antibody at 3.8 µg/ml dilution followed by AP-staining.
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ARG65293 anti-TPPP / p25 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cerebellum tissue. Antigen Retrieval: Microwaved tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65293 anti-TPPP / p25 antibody at 2 µg/ml dilution followed by HRP-staining.
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ARG65293 anti-TPPP / p25 antibody WB image
Western blot: 35 µg of Human cerebellum lysate (in RIPA buffer) stained with ARG65293 anti-TPPP / p25 antibody at 0.001 µg/ml dilution and incubated at RT for 1 hour.
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ARG65293 anti-TPPP / p25 antibody WB image
Western blot: 35 µg of Mouse (A) and Rat (B) brain lysate (in RIPA buffer) stained with ARG65293 anti-TPPP / p25 antibody at 0.001 µg/ml dilution and incubated at RT for 1 hour.
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ARG65293 anti-TPPP / p25 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65293 anti-TPPP / p25 antibody at 3.75 µg/ml dilution followed by AP-staining.
