ARG42649
anti-TPP1 antibody
anti-TPP1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes TPP1 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | TPP1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 227-261 of Human TPP1. (CAQFLEQYFHDSDLAQFMRLFGGNFAHQASVARVV) |
| 偶联标记 | Un-conjugated |
| 別名 | EC 3.4.14.9; TPP-1; Tripeptidyl-peptidase I; GIG1; Lysosomal pepstatin-insensitive protease; SCAR7; Tripeptidyl aminopeptidase; Tripeptidyl-peptidase 1; CLN2; LPIC; Cell growth-inhibiting gene 1 protein; TPP-I |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HeLa | ||||||
| 实际分子量 | ~ 61 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TPP1 |
| 全名 | tripeptidyl peptidase I |
| 背景介绍 | This gene encodes a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome. [provided by RefSeq, Jul 2008] |
| 生物功能 | Lysosomal serine protease with tripeptidyl-peptidase I activity (PubMed:11054422, PubMed:19038966, PubMed:19038967). May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases (PubMed:11054422, PubMed:19038966, PubMed:19038967). Requires substrates with an unsubstituted N-terminus (PubMed:19038966). [UniProt] |
| 细胞定位 | Lysosome. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. [UniProt] |
| 预测分子量 | 61 kDa |
| 翻译后修饰 | Activated by autocatalytic proteolytical processing upon acidification. N-glycosylation is required for processing and activity. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
-
ARG42649 anti-TPP1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue stained with ARG42649 anti-TPP1 antibody at 1 µg/ml dilution.
-
ARG42649 anti-TPP1 antibody WB image
Western blot: HeLa whole cell lysate stained with ARG42649 anti-TPP1 antibody at 0.5 µg/ml dilution.
