ARG10851
anti-TPBG / 5T4 antibody [B3F1]
anti-TPBG / 5T4 antibody [B3F1] for ELISA,Flow cytometry,Functional study,ICC/IF,Western blot and Mouse
概述
| 产品描述 | Mouse Monoclonal antibody [B3F1] recognizes TPBG / 5T4. The mouse 5T4 monoclonal antibody, clone B3F1, defines the human 5T4 oncofoetal antigen, a highly glycosylated protein expressed by trophoblast and a few specialized adult epithelia. Up-regulation of 5T4 expression in some cancers is associated with poor clinical outcome; overexpression of human 5T4 cDNA in epithelial cells can alter their morphology and motility, supporting a role for such functions in cancer and development. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA, FACS, FuncSt, ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | B3F1 |
| 同位型 | IgG2a |
| 靶点名称 | TPBG / 5T4 |
| 抗原物种 | Mouse |
| 抗原表位 | Distal LRR domain of m5T4 |
| 偶联标记 | Un-conjugated |
| 別名 | 5T4 oncofetal trophoblast glycoprotein; WAIF1; 5T4; Trophoblast glycoprotein; 5T4AG; 5T4 oncofetal antigen; M6P1; 5T4 oncotrophoblast glycoprotein; Wnt-activated inhibitory factor 1 |
应用说明
| 应用建议 |
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| 应用说明 | Functional study: This antibody inhibits the chemotactic migration towards CXCL12 exhibited by differentiating WT-ES cells. IC50 for the maximally inhibitory mAb B3F1 was 2.2 µg±0.8. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS and 0.02% Sodium azide. |
| 抗菌剂 | 0.02% Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TPBG |
| 全名 | trophoblast glycoprotein |
| 背景介绍 | This gene encodes a leucine-rich transmembrane glycoprotein that may be involved in cell adhesion. The encoded protein is an oncofetal antigen that is specific to trophoblast cells. In adults this protein is highly expressed in many tumor cells and is associated with poor clinical outcome in numerous cancers. Alternate splicing in the 5' UTR results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2009] |
| 生物功能 | May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization. [UniProt] |
| 预测分子量 | 46 kDa |
| 翻译后修饰 | Highly glycosylated. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG10851 anti-TPBG / 5T4 antibody [B3F1] WB image
Western blot: Recombinant m5T4-pIg stained with ARG10851 anti-TPBG / 5T4 antibody [B3F1].
