ARG56183
anti-TOP1MT antibody [TOP1MT/488]
anti-TOP1MT antibody [TOP1MT/488] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [TOP1MT/488] recognizes TOP1MT |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | TOP1MT/488 |
| 同位型 | IgG2b, kappa |
| 靶点名称 | TOP1MT |
| 抗原物种 | Human |
| 抗原 | Recombinant full-length human TOP1MT protein. |
| 偶联标记 | Un-conjugated |
| 別名 | TOP1mt; EC 5.99.1.2; DNA topoisomerase I, mitochondrial |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Jurkat | ||||||||
| 实际分子量 | ~ 57 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 0.1 mg/ml BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q969P6 Human DNA topoisomerase I, mitochondrial |
|---|---|
| 基因名称 | TOP1MT |
| 全名 | topoisomerase (DNA) I, mitochondrial |
| 背景介绍 | This gene encodes a mitochondrial DNA topoisomerase that plays a role in the modification of DNA topology. The encoded protein is a type IB topoisomerase and catalyzes the transient breaking and rejoining of DNA to relieve tension and DNA supercoiling generated in the mitochondrial genome during replication and transcription. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012] |
| 生物功能 | Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). [UniProt] |
| 细胞定位 | Cytoplasmic (mitochondria) |
| 预测分子量 | 70 kDa |
检测图片 (5) Click the Picture to Zoom In
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ARG56183 anti-TOP1MT antibody [TOP1MT/488] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human gallbladder stained with ARG56183 anti-TOP1MT antibody [TOP1MT/488].
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ARG56183 anti-TOP1MT antibody [TOP1MT/488] WB image
Western blot: Jurkat cell lysate stained with ARG56183 anti-TOP1MT antibody [TOP1MT/488].
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ARG56183 anti-TOP1MT antibody [TOP1MT/488] FACS image
Flow Cytometry: PFA-fixed MCF7 cells stained with ARG56183 anti-TOP1MT antibody [TOP1MT/488] (blue); Isotype control (red).
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ARG56183 anti-TOP1MT antibody [TOP1MT/488] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human melanoma stained with ARG56183 anti-TOP1MT antibody [TOP1MT/488].
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ARG56183 anti-TOP1MT antibody [TOP1MT/488] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human pancreas stained with ARG56183 anti-TOP1MT antibody [TOP1MT/488].
