anti-TIM3 antibody [SQab22265]
anti-TIM3 antibody [SQab22265] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Immune System antibody
|产品描述||Recombinant Rabbit Monoclonal antibody [SQab22265] recognizes TIM3|
|应用||FACS, IHC-P, WB|
|抗原||A synthetic peptide corresponding to the sequence of Human TIM-3.|
|Protein Full name||Hepatitis A virus cellular receptor 2|
|別名||KIM-3; TIM3; TIMD-3; Tim-3; TIM-3; CD366; T-cell immunoglobulin and mucin domain-containing protein 3; TIMD3; T-cell membrane protein 3; HAVcr-2; T-cell immunoglobulin mucin receptor 3; Hepatitis A virus cellular receptor 2|
|应用说明||IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). Incubate the samples at RT (18-25°C) for 30 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|纯化||Purification with Protein A.|
|缓冲液||PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA.|
|抗菌剂||0.01% Sodium azide|
|稳定剂||40% Glycerol and 0.05%BSA|
|存放说明||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|注意事项||For laboratory research only, not for drug, diagnostic or other use.|
|全名||hepatitis A virus cellular receptor 2|
|背景介绍||The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. [provided by RefSeq, Sep 2011]|
|生物功能||Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9. [UniProt]|
Anti-Rabbit IgG secondary antibodies;
New PD-1 ELISA Kit, excellent for preclinical studies or pharmatheutical development
|研究领域||Immune System antibody|
|翻译后修饰||O-glycosylated with core 1 or possibly core 8 glycans.
Phosphorylated on tyrosine residues; modestly increased after TCR/CD28 stimulation. Can be phosphorylated in the cytoplasmatic domain by FYN (By similarity). Phosphorylation at Tyr-265 is increased by stimulation with ligand LGALS9.
检测图片 (3) Click the Picture to Zoom In
ARG66922 anti-TIM3 antibody [SQab22265] WB image (Customer's Feedback)
Western blot: NK92-MI; TIM3 KD NK92-MI and Primary T cell stained with ARG66922 anti-TIM3 antibody [SQab22265] at 1:2000 dilution for 1 hour at room temperature.
ARG66922 anti-TIM3 antibody [SQab22265] FACS image (Customer's Feedback)
Flow Cytometry: NK92-MI; TIM3 KD NK92-MI and Primary T cell stained with ARG66922 anti-TIM3 antibody [SQab22265] at 1:200 dilution for 50 min at 4ºC.
ARG66922 anti-TIM3 antibody [SQab22265] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human tonsil stained with ARG66922 anti-TIM3 antibody [SQab22265]. Antigen Retrieval: Heat tissue section in Tris-EDTA buffer (pH 9.0).
anti-TIM3 antibody [SQab22265]
Sample：Primary T cell; NK92-MI; TIM3 KD NK92-MI
Cell Number/Concentration：5x10^5 cells
Fixation Temperature：- ºC
Primary Antibody Dilution Factor：1:200
Primary Antibody Incubation Time：50 min
Primary Antibody Incubation Temperature：4 ºC