anti-TIM3 antibody [SQab22265]

anti-TIM3 antibody [SQab22265] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Immune System antibody


产品描述 Recombinant Rabbit Monoclonal antibody [SQab22265] recognizes TIM3
反应物种 Hu
宿主 Rabbit
克隆 Monoclonal
克隆号 SQab22265
同位型 IgG
靶点名称 TIM3
抗原物种 Human
抗原 A synthetic peptide corresponding to the sequence of Human TIM-3.
偶联标记 Un-conjugated
Protein Full name Hepatitis A virus cellular receptor 2
別名 KIM-3; TIM3; TIMD-3; Tim-3; TIM-3; CD366; T-cell immunoglobulin and mucin domain-containing protein 3; TIMD3; T-cell membrane protein 3; HAVcr-2; T-cell immunoglobulin mucin receptor 3; Hepatitis A virus cellular receptor 2


应用 推荐稀释比
FACS1:100 - 1:200 (5 x 10^5 cells)
IHC-P1:100 - 1:200
WB1:1000 - 1:3000
应用说明 IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). Incubate the samples at RT (18-25°C) for 30 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Human tonsil


形式 Liquid
纯化 Purification with Protein A.
缓冲液 PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA.
抗菌剂 0.01% Sodium azide
稳定剂 40% Glycerol and 0.05%BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.



GeneID: 84868 Human HAVCR2

Swiss-port # Q8TDQ0 Human Hepatitis A virus cellular receptor 2

基因名称 HAVCR2
全名 hepatitis A virus cellular receptor 2
背景介绍 The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. [provided by RefSeq, Sep 2011]
生物功能 Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9. [UniProt]
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研究领域 Immune System antibody
预测分子量 33 kDa
翻译后修饰 O-glycosylated with core 1 or possibly core 8 glycans.
Phosphorylated on tyrosine residues; modestly increased after TCR/CD28 stimulation. Can be phosphorylated in the cytoplasmatic domain by FYN (By similarity). Phosphorylation at Tyr-265 is increased by stimulation with ligand LGALS9.

检测图片 (3) Click the Picture to Zoom In

  • ARG66922 anti-TIM3 antibody [SQab22265] WB image (Customer's Feedback)

    Western blot: NK92-MI; TIM3 KD NK92-MI and Primary T cell stained with ARG66922 anti-TIM3 antibody [SQab22265] at 1:2000 dilution for 1 hour at room temperature.

  • ARG66922 anti-TIM3 antibody [SQab22265] FACS image (Customer's Feedback)

    Flow Cytometry: NK92-MI; TIM3 KD NK92-MI and Primary T cell stained with ARG66922 anti-TIM3 antibody [SQab22265] at 1:200 dilution for 50 min at 4ºC.

  • ARG66922 anti-TIM3 antibody [SQab22265] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human tonsil stained with ARG66922 anti-TIM3 antibody [SQab22265]. Antigen Retrieval: Heat tissue section in Tris-EDTA buffer (pH 9.0).


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anti-TIM3 antibody [SQab22265]


Sample:NK92-MI; TIM3 KD NK92-MI; Primary T cell

Sample Loading Amount:35 μg

Primary Antibody Dilution Factor:1:2000

Primary Antibody Incubation Time:1 h

Primary Antibody Incubation Temperature:Room Temperature ºC

nuts_pic      Excellent

anti-TIM3 antibody [SQab22265]


Sample:Primary T cell; NK92-MI; TIM3 KD NK92-MI

Cell Number/Concentration:5x10^5 cells

Fixation Buffer:-

Fixation Time:-

Fixation Temperature:- ºC

Primary Antibody Dilution Factor:1:200

Primary Antibody Incubation Time:50 min

Primary Antibody Incubation Temperature:4 ºC