ARG62642
anti-TID1 antibody [RS11]
anti-TID1 antibody [RS11] for ICC/IF,Western blot and Human
Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody [RS11] recognizes TID1 |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, WB |
| 特异性 | Reacts with both short and long variants of TID1 |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | RS11 |
| 同位型 | IgM, kappa |
| 靶点名称 | TID1 |
| 抗原物种 | Human |
| 抗原 | Recombinant full length protein (Human). |
| 偶联标记 | Un-conjugated |
| 別名 | hTid-1; TID1; DnaJ homolog subfamily A member 3, mitochondrial; DnaJ protein Tid-1; Hepatocellular carcinoma-associated antigen 57; Tumorous imaginal discs protein Tid56 homolog; hTID-1; HCA57 |
应用说明
| 应用说明 | IF: 1: 50-1: 500. WB: 2 - 4ug / ml * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|---|
| 阳性对照 | HeLa cells, Jurka |
属性
| 形式 | Liquid |
|---|---|
| 缓冲液 | 10mM PBS (pH 7.4), 0.2% BSA and 0.09% Sodium azide |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 0.2% BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q96EY1 Human DnaJ homolog subfamily A member 3, mitochondrial |
|---|---|
| 基因名称 | DNAJA3 |
| 全名 | DnaJ (Hsp40) homolog, subfamily A, member 3 |
| 背景介绍 | TID-1 is a 52 kDa protein. The human TID-1 gene encodes two splice variants, TID-1L and TID-1S. TID-1L and TID-1S are localized to the mitochon-drial matrix where they regulate apoptotic signal transduction by affectingcytochrome c release and caspase-3 activation. Both TID-1L and TID-1S are cleaved at amino acid 66 upon entry into the mitochondria, indicating thatmature TID-1L and TID-1S represent cleavage products of cytoplasmic pre-proteins. |
| 生物功能 | Modulates apoptotic signal transduction or effector structures within the mitochondrial matrix. Affect cytochrome C release from the mitochondria and caspase 3 activation, but not caspase 8 activation. Isoform 1 increases apoptosis triggered by both TNF and the DNA-damaging agent mytomycin C; in sharp contrast, isoform 2 suppresses apoptosis. Can modulate IFN-gamma-mediated transcriptional activity. Isoform 2 may play a role in neuromuscular junction development as an effector of the MUSK signaling pathway. [UniProt] |
| 细胞定位 | Mitochondria |
| 研究领域 | Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 52 kDa |
| 翻译后修饰 | Tyrosine phosphorylated. |
