ARG41126
anti-TGM3 / TGE antibody
anti-TGM3 / TGE antibody for ICC/IF,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes TGM3 / TGE |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | TGM3 / TGE |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-180 of Human TGM3 (NP_003236.3). |
| 偶联标记 | Un-conjugated |
| 別名 | Protein-glutamine gamma-glutamyltransferase E; E; Transglutaminase-3; TGE; TGase E; TGase-3; Transglutaminase E; TG; EC 2.3.2.13 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | BT474 | ||||||
| 实际分子量 | 72 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q08188 Human Protein-glutamine gamma-glutamyltransferase E Swiss-port # Q08189 Mouse Protein-glutamine gamma-glutamyltransferase E |
|---|---|
| 基因名称 | TGM3 |
| 全名 | transglutaminase 3 |
| 背景介绍 | Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene consists of two polypeptide chains activated from a single precursor protein by proteolysis. The encoded protein is involved the later stages of cell envelope formation in the epidermis and hair follicle. [provided by RefSeq, Jul 2008] |
| 生物功能 | Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold (By similarity). In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath. [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 预测分子量 | 77 kDa |
| 翻译后修饰 | Activated by proteolytic processing. In vitro activation is commonly achieved by cleavage with dispase, a neutral bacterial protease. Dispase cleavage site was proposed to lie between Ser-470 and Ser-471 (PubMed:8099584) or between Pro-465 and Phe-466 (PubMed:16565075). Physiological activation may be catalyzed by CTSL and, to a lesser extent, by CTSS, but not by CTSB, CTSD nor CTSV (PubMed:16565075). [UniProt] |
检测图片 (2) Click the Picture to Zoom In
-
ARG41126 anti-TGM3 / TGE antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG41126 anti-TGM3 / TGE antibody.
-
ARG41126 anti-TGM3 / TGE antibody WB image
Western blot: 25 µg of BT474 cell lysate stained with ARG41126 anti-TGM3 / TGE antibody at 1:1000 dilution.
